...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Plant Science >Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco
【24h】

Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco

机译：玉米 psbA 基因的过表达通过调节烟草中的抗氧化系统，光合能力和胁迫防御基因表达，增强了耐旱性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The psbA (encoding D1 protein) plays an important role in protecting photosystem II (PSII) from oxidative damage in higher plants. In our previous study, the role of the psbA from maize ( Zea mays . L) in response to SO_(2)stress was characterized. To date, information about the involvement of the psbA gene in drought response is scarce. Here we found that overexpression (OE) of ZmpsbA showed increased D1 protein abundance and enhanced drought stress tolerance in tobacco. The drought-tolerant phenotypes of the OE lines were accompanied by increases of key antioxidant enzymes SOD, CAT, and POD activities, but decreases of hydrogen peroxide, malondialdehyde, and ion leakage. Further investigation showed that the OE plants had much less reductions than the wild-type in the net photosynthesis rate (Pn), stomatal conductance (Gs), and the maximal photochemical efficiency of PSII (Fv/Fm) during drought stress; indicating that OE of ZmpsbA may alleviate photosynthesis inhibition during drought. qRT-PCR analysis revealed that there was significantly increased expression of NtLEA5, NtERD10C, NtAREB , and NtCDPK2 in ZmpsbA -OE lines. Together, our results indicate that ZmpsbA improves drought tolerance in tobacco possibly by alleviating photosynthesis reduction, reducing reactive oxygen species accumulation and membrane damage, and modulating stress defense gene expression. ZmpsbA could be exploited for engineering drought-tolerant plants in molecular breeding of crops.
机译：psbA（编码D1蛋白）在保护光系统II（PSII）免受高等植物的氧化损伤中起重要作用。在我们先前的研究中，表征了来自玉米（Zea mays。L）的psbA在响应SO_（2）胁迫中的作用。迄今为止，关于psbA基因参与干旱反应的信息很少。在这里，我们发现ZmpsbA的过表达（OE）显示了烟草中D1蛋白丰度的增加和干旱胁迫耐受性的增强。 OE系的耐旱表型伴随着关键抗氧化剂酶SOD，CAT和POD活性的增加，但过氧化氢，丙二醛和离子泄漏的减少。进一步的研究表明，在干旱胁迫下，OE植物的净光合速率（Pn），气孔导度（Gs）和PSII的最大光化学效率（Fv / Fm）的减少量远低于野生型。提示ZmpsbA的OE可能减轻干旱期间的光合作用抑制。 qRT-PCR分析显示ZmpsbA -OE株中NtLEA5，NtERD10C，NtAREB和NtCDPK2的表达显着增加。总之，我们的结果表明ZmpsbA可能通过减轻光合作用的减少，减少活性氧的积累和膜损伤以及调节逆境防御基因的表达来提高烟草的耐旱性。 ZmpsbA可用于在作物分子育种中工程化耐旱植物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号