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首页> 外文期刊>Majallah-i pizishki-i Urumiyah. >Detection of virulence genes in Escherichia coli strains isolated from pediatric with urinary tract infection and their antibiotic resistance profile
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Detection of virulence genes in Escherichia coli strains isolated from pediatric with urinary tract infection and their antibiotic resistance profile

机译：儿科感染尿路感染大肠杆菌中毒力基因的检测及其抗生素耐药性

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Background: Urinary tract infections (UTI) are one of the most common infections worldwide. Uropathogenic Escherichia coli (UPEC), which causes urinary tract infections are the primary pathogens. The aim of this study is to identify different genes papC, papG, fimH, iutA, sfaS, ibeA and antibiotic susceptibility of E. coli strains isolated from urinary tract infection. Methods : In this study cross-sectional, 120 samples were collected from patients with urinary tract infection referred to children medical Center in Tehran, Iran. Antibiotic susceptibility testing by disk diffusion method was performed according to CLSI guidelines. Thus, DNA extraction was performed from all strains and multiplex PCR was conducted for detection papC, papG, fimH, iutA, sfaS and ibeA virulance genes in all strains. Results: The results showed that E.coli isolates to amikacin (100%) sensitive and had resistance to ampicillin (60%). Virulence genes prevalence was fimH 85%, papC 58%, papG 47%, iutA 35%, sfaS 1% and ibeA 1 %. Conclusion: The results of this study showed that the most common gene encoding genes fimH and papC adhesion genes in E.coli was isolated from urinary tract infection Fimbriae pap. The difference between the results with other studies due to the diversity of geographic region.
机译：背景：尿路感染（UTI）是世界上最常见的感染之一。引起尿路感染的致病性大肠杆菌（UPEC）是主要病原体。这项研究的目的是鉴定从尿路感染分离的大肠杆菌菌株的不同基因papC，papG，fimH，iutA，sfaS，ibeA和抗生素敏感性。方法：在本研究的横断面中，从伊朗德黑兰儿童医疗中心转诊的尿路感染患者中收集了120个样本。根据CLSI指南，通过圆盘扩散法进行了抗生素敏感性测试。因此，从所有菌株中进行DNA提取，并进行多重PCR以检测所有菌株中的papC，papG，fimH，iutA，sfaS和ibeA病毒基因。结果：结果表明，大肠杆菌对阿米卡星敏感（100％），对氨苄西林耐药（60％）。毒力基因的流行率为fimH 85％，papC 58％，papG 47％，iutA 35％，sfaS 1％和ibeA 1％。结论：本研究结果表明，从尿道感染的飞虱中分离出大肠杆菌中最常见的编码fimH和papC粘附基因的基因。由于地理区域的差异，结果与其他研究之间的差异。

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