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首页> 外文期刊>MicrobiologyOpen >Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii
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Identification and characterization of a novel Fusobacterium nucleatum adhesin involved in physical interaction and biofilm formation with Streptococcus gordonii

机译：鉴定和鉴定一种新型的与链球菌物理相互作用和生物膜形成有关的核梭状芽胞粘附素

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Abstract To successfully colonize the oral cavity, bacteria must directly or indirectly adhere to available oral surfaces. Fusobacterium nucleatum plays an important role in oral biofilm community development due to its broad adherence abilities, serving as a bridge between members of the oral biofilm that cannot directly bind to each other. In our efforts to characterize the molecular mechanisms utilized by F. nucleatum to physically bind to key members of the oral community, we investigated the involvement of F. nucleatum outer membrane proteins in its ability to bind to the pioneer biofilm colonizer, Streptococcus gordonii . Here, we present evidence that in addition to the previously characterized fusobacterial adhesin RadD, the interaction between F. nucleatum ATCC 23726 and S. gordonii V288 involves a second outer membrane protein, which we named c oaggregation m ediating p rotein A (CmpA). We also characterized the role of CmpA in dual-species biofilm formation with S. gordonii V288, evaluated growth-phase-dependent as well as biofilm expression profiles of radD and cmpA , and confirmed an important role for CmpA, especially under biofilm growth conditions. Our findings underscore the complex set of specific interactions involved in physical binding and thus community integration of interacting bacterial species. This complex set of interactions could have critical implications for the formation and maturation of the oral biofilms in vivo, and could provide clues to the mechanism behind the distribution of organisms inside the human oral cavity.
机译：摘要为了成功地在口腔中定殖，细菌必须直接或间接附着在可用的口腔表面上。核梭菌由于其广泛的粘附能力而在口腔生物膜群落的发展中起着重要的作用，充当口腔生物膜彼此之间不能直接结合的成员之间的桥梁。在我们努力表征核实镰刀菌与口腔社区关键成员物理结合的分子机制的过程中，我们调查了核实镰刀菌外膜蛋白在结合先驱生物膜定居者戈登链球菌的能力中的参与。在这里，我们提供的证据表明，除了先前表征的融合细菌粘附素RadD之外，核镰刀菌ATCC 23726和戈登氏葡萄球菌V288之间的相互作用还涉及第二种外膜蛋白，我们将其命名为c聚合蛋白A（CmpA）。我们还表征了CmpA在戈氏链球菌V288的双物种生物膜形成中的作用，评估了radD和cmpA的生长阶段依赖性以及生物膜表达谱，并确认了CmpA的重要作用，尤其是在生物膜生长条件下。我们的发现强调了涉及物理结合并因此相互作用的细菌物种的社区整合的一系列复杂的特定相互作用。这种复杂的相互作用可能对体内口腔生物膜的形成和成熟具有关键意义，并可能为人类口腔内部生物分布的背后机制提供线索。

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