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首页> 外文期刊>Molecular oral microbiology >Transcriptional responses of Streptococcus gordonii Streptococcus gordonii and Fusobacterium nucleatum Fusobacterium nucleatum to coaggregation
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Transcriptional responses of Streptococcus gordonii Streptococcus gordonii and Fusobacterium nucleatum Fusobacterium nucleatum to coaggregation

机译：链球菌的转录反应戈德昔尼链球菌和核杆菌核心骨杆菌与共ggregation

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摘要

Cell‐cell interactions between genetically distinct bacteria, known as coaggregation, are important for the formation of mixed‐species biofilms such as dental plaque. Interactions lead to gene regulation in the partner organisms that may be critical for adaptation and survival in mixed‐species biofilms. Here, gene regulation responses to coaggregation between Streptococcus gordonii and Fusobacterium nucleatum were studied using dual RNA‐Seq. Initially, S. ? gordonii was shown to coaggregate strongly with F.?nucleatum in buffer or human saliva. Using confocal laser scanning microscopy and transmission electron microscopy, cells of different species were shown to be evenly distributed throughout the coaggregate and were closely associated with one another. This distribution was confirmed by serial block face sectioning scanning electron microscopy, which provided high resolution three‐dimensional images of coaggregates. Cell‐cell sensing responses were analysed 30?minutes after inducing coaggregation in human saliva. By comparison with monocultures, 16 genes were regulated following coaggregation in F.?nucleatum whereas 119 genes were regulated in S.?gordonii . In both species, genes involved in amino acid and carbohydrate metabolism were strongly affected by coaggregation. In particular, one 8‐gene operon in F.?nucleatum encoding sialic acid uptake and catabolism was up‐regulated 2‐ to 5‐fold following coaggregation. In S.?gordonii , a gene cluster encoding functions for phosphotransferase system‐mediated uptake of lactose and galactose was down‐regulated up to 3‐fold in response to coaggregation. The genes identified in this study may play key roles in the development of mixed‐species communities and represent potential targets for approaches to control dental plaque accumulation.
机译：遗传下不同细菌之间的细胞 - 细胞相互作用，称为共grgation，对于形成混合物种生物膜，如牙菌斑。相互作用导致基因调节在伴侣生物中可能对混合物种生物膜中的适应和生存至关重要。这里，使用双RNA-SEQ研究了链球菌与核心核核心核核心核核心核核心的基因调节响应。最初，S.？戈登蒂被证明与缓冲或人唾液中的F.Nucleatum强烈共同共用。使用共聚焦激光扫描显微镜和透射电子显微镜，显示不同物种的细胞在整个共同聚焦过程中均匀分布，彼此密切相关。通过串行块面部切片扫描电子显微镜确认该分布，其提供了高分辨率的共grongegates的三维图像。在诱导人类唾液中诱导共ggrogation后，分析细胞 - 细胞感测响应30？分钟。通过与单一栽培作用的比较，在F.? Nucleatum的共ggration中调节16个基因，而119个基因在S.?gordonii中受到调节。在这两个种类中，参与氨基酸和碳水化合物代谢的基因受到共ggregation的强烈影响。特别是，在共聚后，编码唾液酸摄取和分解代谢的F.? Nucleatum中的一个8-基因操纵子。在S.?Gordonii中，磷酸转移酶系统介导的乳糖和半乳糖摄取的基因簇编码功能响应于共grgatation被下调至3倍。本研究中鉴定的该基因可能在混合物种社区的发展中起关键作用，并且代表控制牙菌斑堆积的方法的潜在目标。

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