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首页> 外文期刊>Modern Pathology >Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas
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Expression of Pituitary Homeo Box 1 (Ptx1) in Human Non-Neoplastic Pituitaries and Pituitary Adenomas

机译：垂体同源盒1（Ptx1）在人类非肿瘤性垂体和垂体腺瘤中的表达。

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We investigated the localization of pituitary homeo box 1 (Ptx1) protein in five human non-neoplastic pituitaries and 73 of all types of pituitary adenomas using immunohistochemistry, and the expression of Ptx1 messenger RNA (mRNA) in 18 representative pituitary adenomas using the reverse transcriptase polymerase chain reaction (RT-PCR) technique. By immunohistochemical analysis, Ptx1 protein was extensively detected in the nuclei of normal human pituitary cells. Ptx1 was detected in 10/14 (71.4%) of growth hormone (GH)-secreting adenomas, 12/12 (100%) of prolactin (PRL)-secreting adenomas, 18/20 (90%) of adrenocorticotropic hormone (ACTH)-secreting adenomas, 6/7 (85.7%) of thyroid-stimulating hormone (TSH)-secreting adenomas, and 17/20 (85%) of clinically non-functioning adenomas, including 9/10 (90%) of gonadotropin-subunit-positive adenomas. Thus, there was no relationship between Ptx1 expression and a particular type of pituitary adenomas. By RT-PCR analysis, Ptx1 mRNA was expressed in all 18 cases of pituitary adenomas, including two cases negative for Ptx1 protein by immunohistochemistry. These results suggested that Ptx1 may be an universal transcription factor in both neoplastic and non-neoplastic conditions in human pituitaries. The synergistic action with other transcription factors may be speculated to determine the specific production of the anterior pituitary hormones.
机译：我们使用免疫组织化学方法研究了垂体同源盒1（Ptx1）蛋白在5个人类非肿瘤性垂体和73种所有类型的垂体腺瘤中的定位，并使用逆转录酶在18种代表性垂体腺瘤中表达了Ptx1信使RNA（mRNA）。聚合酶链反应（RT-PCR）技术。通过免疫组织化学分析，在正常人垂体细胞的核中广泛检测到Ptx1蛋白。在分泌生长激素（GH）的腺瘤的10/14（71.4％），分泌催乳激素（PRL）的腺瘤的12/12（100％），促肾上腺皮质激素（ACTH）的18/20（90％）中检测到Ptx1分泌腺瘤，6/7（85.7％）促甲状腺激素（TSH）分泌腺瘤和17/20（85％）临床无功能的腺瘤，包括9/10（90％）的促性腺激素亚基阳性腺瘤。因此，Ptx1表达与特定类型的垂体腺瘤之间没有关系。通过RT-PCR分析，Ptx1 mRNA在18例垂体腺瘤中均表达，其中2例经免疫组织化学检测为Ptx1蛋白阴性。这些结果表明Ptx1可能是人类垂体肿瘤和非肿瘤情况下的通用转录因子。可以推测与其他转录因子的协同作用以确定垂体前叶激素的特定产生。

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