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首页> 外文期刊>Nepal Journal of Biotechnology >Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana
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Evaluation of Phytochemical, Antimicrobial, Antioxidant Activity and Cytotoxic Potentials of Agave americana

机译：龙舌兰的植物化学，抗微生物，抗氧化活性和细胞毒性潜力的评估

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Ethnomedicinal plants are being used as a source of medicine from ancient time but they lack theproof of modern scientific evidence for their effectiveness. This study focuses on the evaluation ofphytochemical, antimicrobial, antioxidant properties of one of the ethnomedicinal plant Agaveamericana from Dhulikhel region of Nepal. The plant extract was prepared using solvent-basedwarm soxhlet extraction from the leaves of the plant and antimicrobial activity against sixdifferent non-resistant clinical isolates of bacteria (Staphylococcus aureus, Shigella, Klebsiellapneumoniae, Escherichia coli, Bacillus thuringiensis, and Salmonella paratyphi) was evaluated usingagar disc diffusion method along with qualitative analysis for presence/absence ofphytochemicals. Antioxidant activity was measured by DPPH assay and the cytotoxicity wasevaluated using MCF-7 (human breast adenocarcinoma) cancer cell. Presence of phytochemicalslike alkaloids, flavonoids, reducing sugars and saponins were detected in the plant extract. Theextract was found to show some level of antimicrobial activity against Staphylococcus aureus,Escherichia coli and Bacillus thuringiensis at 50, 100 and 200 mg/ml. The IC50 value of the extractwas found to be 7.68 μg/ml. The extracts of Agave americana showed 50 % cell-death of MCF-7 in12 h at 5 μg/ml. Although this study provided some scientific evidence for the medicinal value ofAgave americana, further studies are still needed for the detailed evaluations of every moleculepresent in this plant along with screening in larger geographical area of Nepal.
机译：药用植物自古以来就被用作药物来源，但它们缺乏有效的现代科学证据。这项研究的重点是评估尼泊尔Dhulikhel地区的一种民族药用植物Agaveamericana的植物化学，抗微生物，抗氧化特性。使用从植物叶片中提取的基于溶剂的温索氏提取物制备植物提取物，并使用琼脂评估其对六种不同的非耐药性细菌分离株（金黄色葡萄球菌，志贺氏菌，克雷伯氏肺炎杆菌，大肠杆菌，苏云金芽孢杆菌和副伤寒沙门氏菌）的抗菌活性。圆盘扩散法和定性分析是否存在植物化学物质。通过DPPH测定法测定抗氧化活性，并使用MCF-7（人乳腺腺癌）癌细胞评价其细胞毒性。在植物提取物中检测到植物化学物质如生物碱，类黄酮，还原糖和皂苷的存在。发现该提取物以50、100和200 mg / ml显示出对金黄色葡萄球菌，大肠杆菌和苏云金芽孢杆菌的一定水平的抗菌活性。提取物的IC 50值为7.68μg/ ml。龙舌兰提取物在12 h内以5μg/ ml的浓度显示出50％的MCF-7细胞死亡。尽管该研究为龙舌兰的药用价值提供了一些科学证据，但仍需要进一步研究以详细评估该植物中存在的每种分子，并在尼泊尔较大的地理区域进行筛选。

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