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首页> 外文期刊>Nucleus >The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation
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The F-actin severing protein cofilin-1 is required for RNA polymerase II transcription elongation

机译：RNA聚合酶II转录延伸需要F-肌动蛋白切断蛋白cofilin-1

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In mammals actin contributes to transcription elongation by facilitating establishment of permissive chromatin. Here we report that the F-actin severing protein cofilin-1 is part of the same complex with actin and phosphorylated RNA polymerase (pol) II. In chromatin immunoprecipitation assays cofilin-1 was found selectively associated with transcribed regions of active genes, its occupancy being influenced by the polymerization state of actin. Cofilin-1 gene silencing led to a drop in FUrd incorporation into nascent transcripts. In cofilin-1 silenced cells chromatin immunoprecipitations showed that active genes were devoid of actin, phosphorylated pol II and displayed low histone H3 acetylation levels on K9. These findings suggest that cofilin-1 plays a major role in pol II transcription, facilitating association of elongating pol II and actin with active genes. We speculate that cofilin-1 performs its function in pol II transcription by regulating polymerization of gene-associated actin.
机译：在哺乳动物中，肌动蛋白通过促进允许的染色质的建立而有助于转录伸长。在这里我们报告F-肌动蛋白切断蛋白cofilin-1是肌动蛋白和磷酸化RNA聚合酶（pol）II相同复合物的一部分。在染色质免疫沉淀测定中，发现cofilin-1与活性基因的转录区选择性结合，其占有率受肌动蛋白聚合状态的影响。 Cofilin-1基因沉默导致FUrd并入新生转录本中的下降。在cofilin-1沉默的细胞中，染色质免疫沉淀显示活性基因缺乏肌动蛋白，磷酸化的pol II，并且在K9上显示出较低的组蛋白H3乙酰化水平。这些发现表明，cofilin-1在pol II转录中起主要作用，有助于延长pol II和肌动蛋白与活性基因的结合。我们推测cofilin-1通过调节基因相关肌动蛋白的聚合反应在pol II转录中执行其功能。

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