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首页> 外文期刊>FEBS Open Bio >Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice
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Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice

机译：在野生型和脂联素敲除小鼠的共生模型中追踪脂联素的运动

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Adiponectin is exclusively synthesized by adipocytes and exhibits anti-diabetic, anti-atherosclerotic and anti-inflammatory properties. Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis. However, the mechanisms responsible for hypoadiponectinemia remain unclear. Here, we investigated adiponectin movement using hetero parabiosis model of wild type (WT) and adiponectin-deficient (KO) mice. WT mice were parabiosed with WT mice (WT-WT) or KO mice (WT-KO) and adiponectin levels were measured serially up to 63days after surgery. In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7. Circulating adiponectin levels decreased further to the detection limit of assay, and remained low up to day 63. However, adiponectin protein was detected in the adipose tissues of not only the WT partner but also WT-KO mice. In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins. The use of parabiosis experiments shed light on movement of native adiponectin among different tissues such as the state of hypoadiponectinemia in obesity.
机译：脂联素是脂肪细胞专门合成的，具有抗糖尿病，抗动脉粥样硬化和抗炎的特性。低脂联素血症在肥胖个体中与胰岛素抵抗和动脉粥样硬化有关。然而，导致低脂联素血症的机制仍不清楚。在这里，我们使用野生型（WT）和脂联素缺乏（KO）小鼠的异质共生模型研究了脂联素的运动。 WT小鼠与WT小鼠（WT-WT）或KO小鼠（WT-KO）一起进行了生物杀伤，并在术后63天连续测量脂联素水平。在WT-KO共生模型中，WT伴侣的循环脂联素水平迅速下降，另一方面，KO伴侣的循环脂联素水平升高，然后在第7天达到彼此相当的水平。循环脂联素水平进一步下降至检测极限。直到第63天仍保持低水平。但是，不仅在WT伴侣而且在WT-KO小鼠的脂肪组织中都检测到脂联素蛋白。在饮食诱导的肥胖模型中，在饮食诱导的肥胖KO伙伴的脂肪基质血管部分中检测到高脂联素蛋白水平，而其结合蛋白没有变化。共生代谢实验的使用揭示了天然脂联素在不同组织之间的运动，例如肥胖中低脂联素血症的状态。

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