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首页> 外文期刊>FEBS Open Bio >Glutathione peroxidase 4 plays an important role in oxidative homeostasis and wound repair in corneal epithelial cells
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Glutathione peroxidase 4 plays an important role in oxidative homeostasis and wound repair in corneal epithelial cells

机译：谷胱甘肽过氧化物酶4在角膜上皮细胞的氧化稳态和伤口修复中起重要作用

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Oxidative stress is involved in the pathologies of corneal epithelial cells. However, the importance of specific antioxidant enzymes in corneal epithelial cells is not fully understood. The purpose of this study is to elucidate the role of glutathione peroxidase 4 (GPx4) in corneal epithelial cells. For in vitro experiments, an immortalized human corneal epithelial cell line was used. Cytotoxicity measured through LDH activity, lipid peroxidation immunostained for 4‐hydroxynonenal, cell viability, and cell death were compared between cells transfected with either GPx4 siRNA or scrambled control siRNA. In addition, the rescue effects of α‐tocopherol and ferrostatin‐1, a ferroptosis inhibitor, were examined in the cells with deficient GPx4 expression. For in vivo experiments, we applied n‐heptanol on the cornea of GPx4+/+ and GPx4+/? mice to create corneal epithelial wound. The epithelial defect area size was measured up to 48 h after epithelial wound creation. Knockdown of GPx4 strongly induced cytotoxicity and cell death in human corneal epithelial cells. Cell death induced by GPx4 knockdown was characterized by positive staining for both annexin V and propidium iodide, nuclear translocation of AIF, and without activation of caspase 3, and was rescued by α‐tocopherol and ferrostatin‐1. The delayed wound healing of GPx4 siRNA‐transfected cells were ameliorated by α‐tocopherol in vitro. In addition, loss of one GPx4 allele was sufficient to significantly delay the healing of experimental corneal epithelial wounds in vivo. Our results suggest that the antioxidant enzyme GPx4 plays an important role in oxidative homeostasis, cell survival, and wound healing in corneal epithelial cells.
机译：氧化应激涉及角膜上皮细胞的病理。但是，尚未完全了解角膜上皮细胞中特定抗氧化剂的重要性。这项研究的目的是阐明谷胱甘肽过氧化物酶4（GPx4）在角膜上皮细胞中的作用。对于体外实验，使用了永生化的人角膜上皮细胞系。比较了用GPx4 siRNA或加扰的对照siRNA转染的细胞的LDH活性，对4-羟基壬烯醛免疫染色的脂质过氧化，细胞存活率和细胞死亡的细胞毒性。此外，在GPx4表达不足的细胞中检查了α-生育酚和ferrostatin-1（一种促铁抑病抑制剂）的拯救作用。对于体内实验，我们在GPx4 + / +和GPx4 + /？角膜上使用了正庚醇。小鼠产生角膜上皮伤口。在创上上皮伤口后48小时内测量上皮缺损区域的大小。击倒GPx4强烈诱导人角膜上皮细胞的细胞毒性和细胞死亡。 GPx4敲低诱导的细胞死亡的特征是膜联蛋白V和碘化丙啶均呈阳性染色，AIF核易位，且未激活caspase 3，并通过α-生育酚和ferrostatin-1得以挽救。 α-生育酚可改善GPx4 siRNA转染细胞的伤口愈合延迟。此外，丢失一个GPx4等位基因足以显着延迟体内实验性角膜上皮伤口的愈合。我们的研究结果表明，抗氧化剂GPx4在角膜上皮细胞的氧化稳态，细胞存活和伤口愈合中起着重要作用。

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