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首页> 外文期刊>Reviews of Reproduction >Unequal distribution of 16S mtrRNA at the 2-cell stage regulates cell lineage allocations in mouse embryos
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Unequal distribution of 16S mtrRNA at the 2-cell stage regulates cell lineage allocations in mouse embryos

机译：2细胞阶段16S mtrRNA的不均匀分布调节小鼠胚胎中的细胞谱系分配

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Cell lineage determination during early embryogenesis has profound effects on adult animal development. Pre-patterning of embryos, such as that of Drosophila and Caenorhabditis elegans, is driven by asymmetrically localized maternal or zygotic factors, including mRNA species and RNA binding proteins. However, it is not clear how mammalian early embryogenesis is regulated and what the early cell fate determinants are. Here we show that, in mouse, mitochondrial ribosomal RNAs (mtrRNAs) are differentially distributed between 2-cell sister blastomeres. This distribution pattern is not related to the overall quantity or activity of mitochondria which appears equal between 2-cell sister blastomeres. Like in lower species, 16S mtrRNA is found to localize in the cytoplasm outside of mitochondria in mouse 2-cell embryos. Alterations of 16S mtrRNA levels in one of the 2-cell sister blastomere via microinjection of either sense or anti-sense RNAs drive its progeny into different cell lineages in blastocyst. These results indicate that mtrRNAs are differentially distributed among embryonic cells at the beginning of embryogenesis in mouse and they are functionally involved in the regulation of cell lineage allocations in blastocyst, suggesting an underlying molecular mechanism that regulates pre-implantation embryogenesis in mouse.
机译：胚胎早期发育过程中细胞谱系的确定对成年动物的发育有深远的影响。诸如果蝇和秀丽隐杆线虫的胚胎的预模式是由不对称定位的母体或合子因子驱动的，包括mRNA种类和RNA结合蛋白。然而，尚不清楚哺乳动物早期胚胎发生如何受到调控以及早期细胞命运决定因素是什么。在这里，我们显示，在小鼠中，线粒体核糖体RNA（mtrRNA）在2细胞姐妹卵裂球之间差异分布。这种分布方式与2细胞姐妹卵裂球之间相等的线粒体的总量或活性无关。与低等物种一样，在小鼠2细胞胚胎中，发现16S mtrRNA定位于线粒体外部的细胞质中。通过显微注射有义或反义RNA改变2细胞姐妹卵裂球之一中16S mtrRNA水平，将其后代驱动到胚泡的不同细胞谱系中。这些结果表明，mtrRNA在小鼠胚胎发生开始时在胚胎细胞中差异分布，并且在功能上参与了胚泡中细胞谱系分配的调节，这提示了调节小鼠植入前胚胎发生的潜在分子机制。

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