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首页> 外文期刊>Revista Brasileira de Fruticultura >Estabelecimento in vitro de porta-enxertos de Prunus sp.
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Estabelecimento in vitro de porta-enxertos de Prunus sp.

机译：李属植物砧木的体外建立。

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摘要

The aim of this work was to establish five Prunus sp. rootstocks on two media cultures. The explants were obtained from mother plants cultivated in greenhouse. Desinfestation was carried out in alcohol and sodium hypochlorid solutions. The experiment was a factorial with five cultivars ('Aldrighi', 'Barrier', 'Capdeboscq', 'Flordaguard' and 'GF677') and two culture media (?MS and QL). The experimental design was of completely randomized blocks, with four replications with five test tubes per plot. A nodal segment was introduced per test tub. The explant culture was maintained at 24 ± 4oC, 20 μE m-2 s-1 and 16 hours photoperiod for four weeks. Independently of culture medium, the rootstocks 'Barrier', 'Capdeboscq' and 'Flordaguard' showed higher in vitro response rate and higher number of shoots. ?MS medium was indicated to 'GF677' establishment and QL medium to 'Aldrighi'. In vitro abnormal plantlets were not observed, suggesting satisfactory conditions to culture. The higher contamination rate was verified in 'Aldrighi' (72%) and 'Capdeboscq' (41%), that are the rootstocks most used in southern Brazil.
机译：这项工作的目的是建立五个李属。两种媒体文化的根源。外植体是从温室栽培的母本植物中获得的。在乙醇和次氯酸钠溶液中进行除臭。该实验是一个析因，具有五个品种（“ Aldrighi”，“ Barrier”，“ Capdeboscq”，“ Flordaguard”和“ GF677”）和两种培养基（？MS和QL）。实验设计是完全随机的块，每块样地重复四次，每个试管五个。每个测试桶都引入一个节段。外植体培养物在24±4oC，20μEm-2 s-1和16小时的光周期下保持4周。独立于培养基，砧木“屏障”，“ Capdeboscq”和“ Flordaguard”显示出更高的体外响应率和更高的芽数。将？MS培养基标记为“ GF677”，将QL培养基标记为“ Aldrighi”。在体外未观察到异常的苗，表明令人满意的培养条件。 “ Aldrighi”（72％）和“ Capdeboscq”（41％）是巴西南部最常用的砧木，因此污染率更高。

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