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首页> 外文期刊>Revista de salud animal >An enzyme-linked immunosorbent assay (ELISA) for serological detection of antibodies against Borrelia in calves in Brazil
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An enzyme-linked immunosorbent assay (ELISA) for serological detection of antibodies against Borrelia in calves in Brazil

机译：酶联免疫吸附试验（ELISA）用于巴西小牛血清学检测抗霍乱弧菌的抗体

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摘要

Bacteria of the genus Borrelia are described as pathogenic agents of several human and animal diseases. An infection nowadays is usually detected via determination of circulating antibodies against Borrelia-specific antigens in body fluids. Thus, the aim of this study was to detect circulating antibodies against Borrelia sp. by enzyme-linked immunosorbent assay (ELISA) in cattle. Seven hundred twenty serum samples from 20 female bovines living in tick-infested areas were collected during three years. Statistical analyses were done by qui-square or Fischer's exact tests, with a confidence level of 95%. For homologous antibodies response to Borrelia sp., the iELISA test showed 93.33% sensitivity and 86.66% specificity. The prevalence of antibodies against Borrelia sp. were 15%, 23.8%, 48.6% and 65.8% at birth, first, second and third year, respectively. The rate of animal showing antibodies to Borrelia sp. indicated the presence of the tickborne spirochaetal agent in the cattle population in the studied region. Therefore, an ELISA could be used to confirm the exposure of cattle to Borrelia sp.
机译：疏螺旋菌属的细菌被描述为几种人类和动物疾病的致病因子。如今，通常通过确定体液中针对疏螺旋体特异性抗原的循环抗体来检测感染。因此，本研究的目的是检测针对疏螺旋体的循环抗体。通过酶联免疫吸附测定（ELISA）在牛中进行。在三年中收集了来自生活在tick虫感染区域的20只雌性牛的720个血清样品。统计分析是通过qui-square或Fischer的精确检验完成的，置信度为95％。对于针对博雷利亚氏菌的同源抗体，iELISA测试显示93.33％的敏感性和86.66％的特异性。普遍存在的抗伯氏疏螺旋体的抗体。分别在出生，第一，第二和第三年分别为15％，23.8％，48.6％和65.8％。动物显示出针对疏螺旋体的抗体的速率。表明在研究区域的牛群中存在壁虱气生螺旋体药剂。因此，ELISA可以用于确认牛暴露于疏螺旋体。

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