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首页> 外文期刊>Revista de salud animal >MATURATION AND FERTILIZATION IN VITRO OF SOW OOCYTES OBTAINED FOR CUT FOLLICLES AND PUNCTURE
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MATURATION AND FERTILIZATION IN VITRO OF SOW OOCYTES OBTAINED FOR CUT FOLLICLES AND PUNCTURE

机译：用于切割卵泡和穿刺的母猪卵母细胞的体外成熟和受精

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摘要

A number of 1331 oocytes were recovered from ovaries obtained in slaughterhouses; from these 670 were obtained by puncture and 661 by follicles cutting, then cultivated in the medium defined suplemented TCM-199. Maturation was evaluated by fixation and staining with acetic orceín from 302 ovocytes fixed by puncture method 97 matured and from 294 oocytes fixed by cutting method 95 matured corresponding 32,1 and 32 from maturation percentage respectively, between both groups. A number 368 oocytes from the puncture method and 367 from the cutting one were fertilized in , TBM buffered medium with Tris and then transfered to the development embryo medium NCSU-23. The obtained results were 40 embryos in those of method of puncture and 36 embryos in cutting method, corresponding 11,8% and 11,2% of embryonic development respectively, not observing any significant statistical difference (P0.05), between both groups. One concludes that the efficiency in both procedures of obtaining of oocytes for maturation in vitro of oocytes of pig and its fertilization in vitro is equal, since they significantly did not affect the percentage in the maturation and embryonic development in vitro.
机译：从屠宰场获得的卵巢中回收了1331个卵母细胞。从这670个中通过穿刺获得，从661个中通过卵泡切开获得，然后在限定的补充TCM-199培养基中培养。两组之间通过穿刺法97固定的302卵母细胞和用切割法95固定的294卵母细胞分别从成熟百分率分别成熟的32,1和32进行醋酸乙酸固定和染色，评估其成熟度。将来自穿刺方法的368卵母细胞和来自切割方法的367卵母细胞在含有Tris的TBM缓冲培养基中受精，然后转移至发育胚胎培养基NCSU-23。两组的结果分别为：穿刺法胚胎40个，cutting割法胚胎36个，分别占胚胎发育的11.8％和11.2％，差异无统计学意义（P> 0.05）。一个结论是，获得猪卵母细胞体外成熟和体外受精的效率均相同，因为它们不会显着影响体外卵母细胞的成熟和胚胎发育的百分比。

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