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首页> 外文期刊>Romanian Journal of Biophysics >INSERTION OF PROTEINS IN THE LIPID BILAYER OF LIPOSOMES REVEALED BY FRET
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INSERTION OF PROTEINS IN THE LIPID BILAYER OF LIPOSOMES REVEALED BY FRET

机译：通过FRET揭示的脂质体脂质层中蛋白质的插入。

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Many processes at the level of biological membranes involve the participation of integral and/or adsorbed proteins. The proper functioning of the membrane proteins is related to the local molecular environment, one of the most important factors being the lipid bilayer. In this paper we present a method to evidence the interaction of proteins with the lipid bilayer in artificial lipid membranes using the fluorescence resonance energy transfer (FRET) method. The donor in the energy transfer process is the tryptophan residue from the proteins and the acceptor is diphenylhexatriene, a lipid bilayer fluorescencent marker. Liposomes prepared from lecitine and synthetic lipids were used as model membranes. The FRET procedure was first tested on gramicidin A inserted in the liposome bilayer and afterwards applied to study the insertion of a bacterial outer membrane porin, namely OmpF. Recording the spectra of liposomes-protein-diphenylhexatriene complex for different concentration of the acceptor, the energy transfer was evidenced and the results were discussed in the terms of FRET efficiency.
机译：生物膜水平的许多过程都涉及完整和/或吸附的蛋白质的参与。膜蛋白的适当功能与局部分子环境有关，最重要的因素之一是脂质双层。在本文中，我们提出了一种使用荧光共振能量转移（FRET）方法来证明蛋白质与人工脂质膜中的脂质双层相互作用的方法。能量转移过程中的供体是蛋白质中的色氨酸残基，受体是脂质双层荧光标记二苯己三烯。由卵磷脂和合成脂质制备的脂质体用作模型膜。 FRET方法首先在插入脂质体双层中的短杆菌肽A上进行测试，然后用于研究细菌外膜孔蛋白即OmpF的插入。记录不同浓度受体的脂质体-蛋白质-二苯基己三烯复合物的光谱，证明了能量转移，并根据FRET效率讨论了结果。

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