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首页> 外文期刊>Romanian Journal of Biophysics >LOW LEVEL LONG WAVELENGTH LASER IRRADIATION EFFECTS ON CELL CYCLE PROGRESSION AND APOPTOSIS OF ENERGY RESTRICTED JURKAT T-CELLS
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LOW LEVEL LONG WAVELENGTH LASER IRRADIATION EFFECTS ON CELL CYCLE PROGRESSION AND APOPTOSIS OF ENERGY RESTRICTED JURKAT T-CELLS

机译：低水平长波激光辐照对能量受限的Jurchat T细胞细胞周期和凋亡的影响

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摘要

We investigated the effects of low power 680 nm far-red (FR) and 830 nm near-infrared (NIR) laser light on viability, survival/proliferation, apoptosis rate, and cell cycle progression of noninjured and energy restricted human acute T leukemic Jurkat cells. Data obtained both by microscopy and by flow cytometry demonstrated significant changes in the explored parameters. Energy restriction induced by blockade of oxidative phosphorylation with low concentrations of cyanide (1 mM NaCN) caused intoxication duration dependent viability and survival rate decrease, and apoptosis induction. NIR laser irradiation increased the percentage of live subpopulation in both non-injured and in cyanide intoxicated cell samples. FR laser light did not offer protection to non-injured Jurkat cells, but increased the percentage of live subpopulation in cyanide intoxicated samples. Cyanide intoxication caused G2/M stage blockade and apoptosis induction in an intoxication duration dependent manner. NIR and FR laser irradiation effects on human T leukemia lymphoblasts distribution in various cell cycle stages, as well as modulation of cyanide induced changes were dependent on cells state, duration of intoxication, and on irradiation dose. Low dose NIR irradiation promoted cell cycle progression, while at higher doses, an increase in percentage of cell subpopulations in G1 and G2 phases, and S-phase blockade with apoptosis promotion were observed in the NIR and FR irradiated non-injured samples, respectively. In same conditions, with first irradiation applied early in the intoxication period, enhancement of the cyanide-induced G2 phase blockade and apoptosis induction occurred in FR irradiated samples, while NIR laser light appeared to cause S-phase cell accumulation in cyanide intoxicated samples.
机译：我们研究了低功率680 nm远红外（FR）和830 nm近红外（NIR）激光对无伤害和能量受限的人类急性T白血病Jurkat的生存力，存活/增殖，凋亡率和细胞周期进程的影响细胞。通过显微镜和流式细胞术获得的数据表明所探查参数的显着变化。低浓度氰化物（1 mM NaCN）阻断氧化磷酸化诱导的能量限制导致中毒持续时间依赖性生存能力和存活率降低，并诱导细胞凋亡。近红外激光辐照增加了未受伤和氰化物中毒的细胞样品中活亚群的百分比。 FR激光无法为未受伤的Jurkat细胞提供保护，但会增加氰化物中毒样品中活亚群的百分比。氰化物中毒以中毒持续时间依赖性方式引起G2 / M期阻滞和细胞凋亡诱导。 NIR和FR激光照射对人T白血病淋巴母细胞在各个细胞周期阶段的分布以及氰化物诱导的变化的调节取决于细胞状态，中毒持续时间和照射剂量。低剂量NIR辐照可促进细胞周期进程，而高剂量时，分别在NIR和FR辐照的未损伤样品中观察到G1和G2期细胞亚群的百分比增加，并且S期阻滞促进细胞凋亡。在相同条件下，在中毒期初期进行首次照射，在FR照射的样品中氰化物诱导的G2相阻滞增强和凋亡诱导，而NIR激光似乎导致氰化物中毒的样品中S期细胞蓄积。

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