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首页> 外文期刊>Onderstepoort Journal of Veterinary Research >Construction and immunogenicity of a ?apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis
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Construction and immunogenicity of a ?apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis

机译：胸膜肺炎放线杆菌和副猪嗜血杆菌的？apxIC / ompP2突变体的构建和免疫原性

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摘要

The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxIactivating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named DapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis . It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD 50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD 50 of the mutant strain and parent strain in mice were determined to be 1.0 × 10 7 CFU and 3.5 × 10 5 CFU respectively). The mutant strain that was attenuated could secrete inactivated ApxIA RTX toxins with complete antigenicity and could be used as a candidate live vaccine strain against infections of A. pleuropneumoniae and H. parasuis.
机译：通过涉及蔗糖反选择的方法删除了编码ApxI激活蛋白的胸膜肺炎放线杆菌血清型5（SC-1）的apxIC基因。在这项研究中，构建了胸膜肺炎链球菌的突变株（SC-1），并将其命名为DapxIC / ompP2。该突变株在副嗜血杆菌ompP2基因的缺失位点含有外源DNA。与亲本菌株相比，它在小鼠中没有溶血活性，并具有较低的细胞毒性，并且还评估了其安全性和免疫原性。 LD 50数据显示，与亲本菌株相比，该突变菌株减毒30倍（确定小鼠中该突变菌株和亲本菌株的LD 50分别为1.0×10 7 CFU和3.5×10 5 CFU）。减毒的突变株可以分泌具有完全抗原性的灭活的ApxIA RTX毒素，并且可以用作针对胸膜肺炎链球菌和副猪嗜血杆菌感染的候选活疫苗株。

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