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首页> 外文期刊>Clinical and vaccine immunology: CVI >The Live Attenuated Actinobacillus pleuropneumoniae Triple-Deletion Mutant ΔapxIC ΔapxIIC ΔapxIV-ORF1 Strain, SLW05, Immunizes Pigs against Lethal Challenge with Haemophilus parasuis
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The Live Attenuated Actinobacillus pleuropneumoniae Triple-Deletion Mutant ΔapxIC ΔapxIIC ΔapxIV-ORF1 Strain, SLW05, Immunizes Pigs against Lethal Challenge with Haemophilus parasuis

机译：活减毒胸膜肺炎放线杆菌三缺失突变体ΔapxICΔapxIICΔapxIV-ORF1株SLW05使猪免受副猪嗜血杆菌的致死性攻击。

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Haemophilus parasuis and Actinobacillus pleuropneumoniae both belong to the family Pasteurellaceae and are major respiratory pathogens that cause large economic losses in the pig industry worldwide. We previously constructed an attenuated A. pleuropneumoniae serovar 1 live vaccine prototype, SLW05 (ΔapxIC ΔapxIIC ΔapxIV-ORF1), which is able to produce nontoxic but immunogenic ApxIA, ApxIIA, and ApxIVA. This triple-deletion mutant strain was shown to elicit protective immunity against virulent A. pleuropneumoniae. In the present study, we investigated whether immunization with SLW05 could also protect against lethal challenge with virulent H. parasuis SH0165 (serovar 5) or MD0322 (serovar 4). The SLW05 strain was found to elicit a strong humoral antibody response in pigs and to confer significant protection against challenge with a lethal dose of H. parasuis SH0165 or MD0322. IgG subtype analysis revealed that SLW05 induces a bias toward a Th1-type immune response and stimulates interleukin 2 (IL-2) and gamma interferon (IFN-γ) production. Moreover, antisera from SLW05-vaccinated pigs efficiently inhibited both A. pleuropneumoniae and H. parasuis growth in a whole-blood assay. This is the first report that a live attenuated A. pleuropneumoniae vaccine with SLW05 can protect against lethal H. parasuis infection, which provides a novel approach for developing an attenuated H. parasuis vaccine.
机译：副猪嗜血杆菌和胸膜肺炎放线杆菌均属于巴斯德杆菌科，是主要的呼吸道病原体，它们在全世界的养猪业中造成巨大的经济损失。我们之前曾构建了减毒的胸膜肺炎链球菌血清型1活疫苗原型SLW05（Δ apxIC Δ apxIIC Δ apxIV - ORF1 ），能够产生无毒但具有免疫原性的ApxIA，ApxIIA和ApxIVA。该三缺失突变株显示出对强力胸膜肺炎链球菌的保护性免疫。在本研究中，我们调查了用SLW05免疫是否还可以抵抗致命的副猪嗜血杆菌SH0165（血清型5）或MD0322（血清型4）的致命攻击。发现SLW05株在猪中引起强烈的体液抗体反应，并赋予致命剂量的副猪嗜血杆菌SH0165或MD0322显着的抗攻击性。 IgG亚型分析显示SLW05诱导了对Th1型免疫反应的偏向，并刺激白介素2（IL-2）和γ干扰素（IFN-γ）的产生。此外，在全血试验中，接种SLW05的猪的抗血清可有效抑制胸膜肺炎链球菌和副猪嗜血杆菌的生长。这是第一个报道，带有SLW05的减毒活胸膜肺炎链球菌活疫苗可以预防致命的副猪嗜血杆菌感染，这为开发减毒副猪嗜血杆菌疫苗提供了一种新方法。

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