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首页> 外文期刊>Open Longevity Science >The Thiamine Analogue and Advanced Glycation Endproducts Crosslink Breaker ALT-711 does not Interfere with Transketolase Activity
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The Thiamine Analogue and Advanced Glycation Endproducts Crosslink Breaker ALT-711 does not Interfere with Transketolase Activity

机译：硫胺类似物和高级糖基化终产物交联破坏剂ALT-711不会干扰转酮醇酶活性

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摘要

The enzyme transketolase (sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glycolaldehydetransferase, EC 2.2.1.1) is involved in the pentose phosphate pathway (PPP) and catalyses the transfer of a 2-carbon fragment from a 5-carbon keto sugar (xylulose-5-P) to a 5-carbon aldo sugar (ribose-5-P) to form a 7-carbon keto sugar (sedoheptulose- 7-P) and a 3-carbon aldo sugar (glyceraldehyde-3-P). Transketolase requires thiamine pyrophosphate as a co-factor. Advanced glycation endproducts (AGEs) are implicated in the complications of diabetes and aging, primarily via adventitious and crosslinking of tissue proteins. ALT-711 is an AGE crosslink breaker and has been tested as an intervention therapy in established complications of diabetes. It has been noticed that it has a similar structure to that of thiamine and it was hypothesized that it might inhibit transketolase by replacing the active co-factor rendering the enzyme inactive. In this study, we have established a novel microtiter plate format transketolase assay which determines the concentration of NADH by measuring its fluorescence. Using this assay, it was found that ALT-711 does not inhibit the activity of transketolase up to concentration of 5 mM. We conclude that ALT-711 does not interfere with transketolase activity at clinically relevant concentrations.
机译：酶转酮醇酶（七庚糖七磷酸：D-甘油醛-3-磷酸乙二醇醛转移酶，EC 2.2.1.1）参与戊糖磷酸途径（PPP），并催化从5碳酮到2碳片段的转移。糖（xylulose-5-P）转化为5碳醛糖（ribose-5-P）形成7碳酮糖（sedoheptulose- 7-P）和3碳醛糖（甘油醛3-P） ）。转酮醇酶需要硫胺素焦磷酸作为辅助因子。晚期糖基化终产物（AGEs）与糖尿病和衰老的并发症有关，主要是组织蛋白的不定和交联。 ALT-711是AGE交联破坏剂，已被测试为确定的糖尿病并发症的介入疗法。已经注意到，它具有与硫胺素相似的结构，并且据推测它可以通过取代使酶失活的活性辅因子而抑制转酮醇酶。在这项研究中，我们建立了一种新型的微量滴定板形式的转酮醇酶测定法，该测定法通过测量其荧光来确定NADH的浓度。使用该测定法，发现直至浓度为5 mM时ALT-711均不抑制转酮醇酶的活性。我们得出结论，在临​​床相关浓度下，ALT-711不会干扰转酮醇酶活性。

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