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首页> 外文期刊>Open Journal of Respiratory Diseases >Comparison of Protein Profiles in Sputum between COPD and Acute Exacerbation of COPD
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Comparison of Protein Profiles in Sputum between COPD and Acute Exacerbation of COPD

机译：慢性阻塞性肺病与慢性阻​​塞性肺病急性加重期痰中蛋白质谱的比较

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Background and objective: Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation that is associated with an abnormal inflammatory response of the lung to noxious particles or gases. Cigarette smoking is the major risk factor for the development of COPD. This study evaluated the levels of cyclophilin B in sputa from patients with COPD and COPD with acute exacerbation (AECOPD). Materials and Methods: Two-dimensional electrophoresis was used for differential display proteomics. Western blotting was used to identify and quantify cyclophilin B in sputum from subjects with AECOPD and COPD. Results: Forty-nine protein spots differed in relative intensity between the AECOPD (n = 6) and COPD (n = 6) subjects. Twenty proteins showed increased expression in the sputum of AECOPD subjects, and 29 proteins were present at lower levels in AECOPD sputum compared with COPD sputum. One of these proteins was associated with cyclophilin B. Cyclophilin B concentrations were lower in sputum from subjects with COPD (n = 4) versus AECOPD (n = 4). Conclusion: The sputum proteomic analysis suggests that changes in various proteins are associated with the development of AECOPD.
机译：背景与目的：慢性阻塞性肺疾病（COPD）的特征是进行性气流受限，这与肺对有害颗粒或气体的异常炎症反应有关。吸烟是发展COPD的主要危险因素。这项研究评估了COPD和COPD急性加重（AECOPD）患者痰液中亲环素B的水平。材料和方法：二维电泳用于差异显示蛋白质组学。 Western印迹法用于鉴定和定量AECOPD和COPD患者痰液中的亲环素B。结果：AECOPD（n = 6）和COPD（n = 6）受试者之间的相对强度有49个蛋白质斑点。与COPD痰相比，二十种蛋白在AECOPD受试者的痰中表达增加，并且29种蛋白在AECOPD痰中的含量较低。这些蛋白之一与亲环蛋白B相关。COPPD患者（n = 4）的痰液中亲环蛋白B浓度低于AECOPD（n = 4）。结论：痰蛋白质组学分析表明，各种蛋白质的变化与AECOPD的发生有关。

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