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首页> 外文期刊>Sao Paulo Medical Journal >Tissue microarrays for testing basal biomarkers in familial breast cancer cases
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Tissue microarrays for testing basal biomarkers in familial breast cancer cases

机译：用于检测家族性乳腺癌病例中基础生物标志物的组织芯片

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CONTEXT AND OBJECTIVE: The proteins p63, p-cadherin and CK5 are consistently expressed by the basal and myoepithelial cells of the breast, although their expression in sporadic and familial breast cancer cases has yet to be fully defined. The aim here was to study the basal immunopro-file of a breast cancer case series using tissue microarray technology. DESIGN AND SETTING: This was a cross-sectional study at Universidade Estadual de Campinas, Brazil, and the Institute of Pathology and Mo-lecular Immunology, Porto, Portugal. METHODS: Immunohistochemistry using the antibodies p63, CK5 and p-cadherin, and also estrogen receptor (ER) and Human Epidermal Receptor Growth Factor 2 (HER2), was per-formed on 168 samples from a breast cancer case series. The criteria for identifying women at high risk were based on those of the Breast Cancer Linkage Consortium. RESULTS: Familial tumors were more frequently positive for the p-cadherin (p = 0.0004), p63 (p < 0.0001) and CK5 (p < 0.0001) than was sporadic cancer. Moreover, familial tumors had coexpression of the basal biomarkers CK5+/ p63+, grouped two by two (OR = 34.34), while absence of coexpression (OR = 0.13) was associ-ated with the sporadic cancer phenotype. CONCLUSION: Familial breast cancer was found to be associated with basal biomarkers, using tissue microarray technology. Therefore, characterization of the familial breast cancer phenotype will improve the understanding of breast carcinogenesis.
机译：上下文和目的：尽管在散发性和家族性乳腺癌病例中它们的表达尚未完全确定，但p63，p-钙粘着蛋白和CK5蛋白在乳腺癌的基底和肌上皮细胞中始终表达。本文的目的是使用组织芯片技术研究乳腺癌病例系列的基础免疫原性。设计与地点：这是巴西巴西坎皮纳斯大学学院和葡萄牙波尔图病理与分子免疫研究所的横断面研究。方法：对来自乳腺癌病例系列的168个样本进行了免疫组织化学分析，使用的抗体包括p63，CK5和p-cadherin抗体，以及雌激素受体（ER）和人类表皮受体生长因子2（HER2）。确定高危妇女的标准是根据乳腺癌联系协会的标准。结果：与散发性癌症相比，家族性肿瘤对p-钙粘蛋白（p = 0.0004），p63（p <0.0001）和CK5（p <0.0001）的阳性率更高。此外，家族性肿瘤具有基础生物标志物CK5 + / p63 +的共表达，被二分为二（OR = 34.34），而缺乏共表达（OR = 0.13）与散发性癌症表型相关。结论：利用组织微阵列技术发现家族性乳腺癌与基础生物标志物有关。因此，家族性乳腺癌表型的表征将提高对乳腺癌致癌作用的理解。

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