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首页> 外文期刊>Sao Paulo Medical Journal >Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
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Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA

机译：白癜风：使用黑素细胞形态和酪氨酸酶mRNA的逆转录酶聚合酶链反应，单独分析嫁接与刮除

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CONTEXT AND OBJECTIVE: Recent studies have indicated that vitiligo areas contain inactive or dormant melanocytes. Melanin synthesis is related to tyrosinase presence and indicative of active metabolic state. The aim of this study was to compare repigmentation, epidermal melanocyte distribution and tyrosinase mRNA detection through reverse transcriptase polymerase chain reaction, in tissue samples of vitiligo, before and after curettage, with or without subsequent autologous skin graft using a new method. DESIGN AND SETTING: Prospective, in the Department of Dermatology, Faculdade de Medicina do ABC, Santo André. METHODS: Two vitiligo areas were curetted. One subsequently received grafted normal sacral autologous skin, whereas the other had no further treatment. The curetted areas were examined after 30 days, to evaluate the degree of repigmentation. The melanocyte percentages and tyrosinase mRNA presence in normal skin and vitiligo areas, before and after curettage and grafting, were compared. RESULTS: Complete repigmentation was seen in all grafted areas, whereas non-grafted curetted vitiligo presented partial repigmentation. The melanocyte percentage in grafted areas was greater than in non-treated vitiligo skin (p = 0.01) and skin with curettage alone (p = 0.015). Tyrosinase mRNA was negative in 93.75% of non-treated vitiligo areas. After treatment (curettage alone or curettage and grafting), all lesions became positive for tyrosinase mRNA. CONCLUSION: Metabolically inactive or dormant melanocytes are probably present within vitiligo areas, and may be activated by exogenous or endogenous stimuli.
机译：背景与目的：最近的研究表明，白癜风区域包含无活性或休眠的黑素细胞。黑色素的合成与酪氨酸酶的存在有关，并指示活跃的代谢状态。这项研究的目的是比较刮除术前后白癜风组织样品中的色素沉着，表皮黑素细胞分布和酪氨酸酶mRNA检测（通过逆转录酶聚合酶链反应），采用或不用新方法进行自体皮肤移植。设计与设置：前瞻性研究机构SantoAndré的Faculdade de Medicina do ABC皮肤科。方法：将两个白癜风区域刮除。一个随后接受了移植的正常graft骨自体皮肤，而另一个没有进一步的治疗。 30天后检查刮匙区域，以评估色素沉着的程度。比较刮除和嫁接前后正常皮肤和白癜风区域的黑色素细胞百分比和酪氨酸酶mRNA的存在。结果：在所有移植区域均可见完全色素沉着，而未移植的刮匙白癜风表现出部分色素沉着。移植区域的黑素细胞百分比高于未经治疗的白癜风皮肤（p = 0.01）和仅具有刮除术的皮肤（p = 0.015）。在未经治疗的白癜风区域，酪氨酸酶mRNA阴性，占93.75％。治疗后（单独刮宫或刮宫和植骨），所有病变的酪氨酸酶mRNA均为阳性。结论：白癜风区域内可能存在代谢失活或休眠的黑素细胞，并可能被外源或内源性刺激激活。

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