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首页> 外文期刊>Oxidative Medicine and Cellular Longevity >The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process
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The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process

机译：年龄对人脂肪基质干细胞（hASCs）成骨和成脂分化潜能的影响以及应力因子在分化过程中的影响

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Human adipose tissue is a great source of autologous mesenchymal stem cells (hASCs), which are recognized for their vast therapeutic applications. Their ability to self-renew and differentiate into several lineages makes them a promising tool for cell-based therapies in different types of degenerative diseases. Thus it is crucial to evaluate age-related changes in hASCs, as the elderly are a group that will benefit most from their considerable potential. In this study we investigated the effect of donor age on growth kinetics, cellular senescence marker levels, and osteogenic and adipogenic potential of hASCs. It also has been known that, during life, organisms accumulate oxidative damage that negatively affects cell metabolism. Taking this into consideration, we evaluated the levels of nitric oxide, reactive oxygen species, and superoxide dismutase activity. We observed that ROS and NO increase with aging, while SOD activity is significantly reduced. Moreover cells obtained from older patients displayed senescence associated features, for example,β-galactosidase activity, enlarged morphology, and p53 protein upregulation. All of those characteristics seem to contribute to decreased proliferation potential of those cells. Our results suggest that due to aging some cellular modification may be required before applying aged cells efficiently in therapies such as tissue engineering and regenerative medicine.
机译：人脂肪组织是自体间充质干细胞（hASCs）的重要来源，自体间充质干细胞因其广泛的治疗应用而闻名。它们具有自我更新和分化成几种谱系的能力，使其成为在不同类型的退行性疾病中进行细胞疗法的有前途的工具。因此，评估与年龄相关的hASCs的变化至关重要，因为老年人是受益于其巨大潜力的群体。在这项研究中，我们调查了供体年龄对hASCs生长动力学，细胞衰老标记水平以及成骨和成脂潜能的影响。还已经知道，在生命中，生物体会积累对细胞代谢产生负面影响的氧化损伤。考虑到这一点，我们评估了一氧化氮，活性氧种类和超氧化物歧化酶活性的水平。我们观察到，ROS和NO随老化而增加，而SOD活性则明显降低。而且，从老年患者获得的细胞显示出与衰老相关的特征，例如，β-半乳糖苷酶活性，扩大的形态和p53蛋白上调。所有这些特征似乎都有助于降低这些细胞的增殖潜能。我们的结果表明，由于衰老，在有效地将衰老的细胞应用于诸如组织工程和再生医学等治疗方法之前，可能需要进行一些细胞修饰。

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