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首页> 外文期刊>Standards in Genomic Sciences >The complete genome sequence of Eubacterium limosum SA11, a metabolically versatile rumen acetogen
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The complete genome sequence of Eubacterium limosum SA11, a metabolically versatile rumen acetogen

机译：代谢通用的瘤胃产醋杆菌Eubacterium limosum SA11的完整基因组序列

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Acetogens are a specialized group of anaerobic bacteria able to produce acetate from COsub2/sub and Hsub2/sub via the Wood–Ljungdahl pathway. In some gut environments acetogens can compete with methanogens for Hsub2/sub, and as a result rumen acetogens are of interest in the development of microbial approaches for methane mitigation. The acetogen Eubacterium limosum SA11 was isolated from the rumen of a New Zealand sheep and its genome has been sequenced to examine its potential application in methane mitigation strategies, particularly in situations where hydrogenotrophic methanogens are inhibited resulting in increased Hsub2/sub levels in the rumen. The 4.15?Mb chromosome of SA11 has an average G?+?C content of 47?%, and encodes 3805 protein-coding genes. There is a single prophage inserted in the chromosome, and several other gene clusters appear to have been acquired by horizontal transfer. These include genes for cell wall glycopolymers, a type VII secretion system, cell surface proteins and chemotaxis. SA11 is able to use a variety of organic substrates in addition to Hsub2/sub/COsub2/sub, with acetate and butyrate as the principal fermentation end-products, and genes involved in these metabolic pathways have been identified. An unusual feature is the presence of 39 genes encoding trimethylamine methyltransferase family proteins, more than any other bacterial genome. Overall, SA11 is a metabolically versatile organism, but its ability to grow on such a wide range of substrates suggests it may not be a suitable candidate to take the place of hydrogen-utilizing methanogens in the rumen.
机译：产乙酸菌是一组专门的厌氧细菌，它们能够通过Wood-Ljungdahl途径从CO 2 和H 2 产生乙酸盐。在某些肠道环境中，产乙酸原菌可以与产甲烷菌竞争H 2 ，因此，瘤胃产乙酸原菌对于开发减少甲烷的微生物方法很有用。从新西兰绵羊的瘤胃中分离了产乙酸优生细菌Eu11，对其基因组进行了测序，以研究其在甲烷减缓策略中的潜在应用，特别是在氢营养型产甲烷菌被抑制导致H 2 瘤胃中的水平。 SA11的4.15ΔMb染色体的平均Gα+ΔC含量为47％，编码3805个蛋白质编码基因。在染色体中插入了一个原噬菌体，其他几个基因簇似乎是通过水平转移获得的。这些包括细胞壁糖聚合物的基因，VII型分泌系统，细胞表面蛋白和趋化性。除了H 2 / CO 2 ，SA11还可以使用多种有机底物，其中乙酸盐和丁酸盐是主要的发酵终产物，并且涉及这些基因已经确定了代谢途径。一个不寻常的特征是存在编码三甲胺甲基转移酶家族蛋白的39个基因，比任何其他细菌基因组都多。总体而言，SA11是一种代谢通用的生物，但其在如此广泛的底物上生长的能力表明，它可能不是替代瘤胃中利用氢的产甲烷菌的合适候选者。

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