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首页> 外文期刊>Pathogens >Metagenomic Characterization of Bacterial Communities on Ready-to-Eat Vegetables and Effects of Household Washing on their Diversity and Composition
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Metagenomic Characterization of Bacterial Communities on Ready-to-Eat Vegetables and Effects of Household Washing on their Diversity and Composition

机译：即食蔬菜上细菌群落的元基因组学表征和家庭洗涤对其多样性和组成的影响

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Ready-to-eat (RTE) leafy salad vegetables are considered foods that can be consumed immediately at the point of sale without further treatment. The aim of the study was to investigate the bacterial community composition of RTE salads at the point of consumption and the changes in bacterial diversity and composition associated with different household washing treatments. The bacterial microbiomes of rocket and spinach leaves were examined by means of 16S rRNA gene high-throughput sequencing. Overall, 886 Operational Taxonomic Units (OTUs) were detected in the salads’ leaves. Proteobacteria was the most diverse high-level taxonomic group followed by Bacteroidetes and Firmicutes. Although they were processed at the same production facilities, rocket showed different bacterial community composition than spinach salads, mainly attributed to the different contributions of Proteobacteria and Bacteroidetes to the total OTU number. The tested household decontamination treatments proved inefficient in changing the bacterial community composition in both RTE salads. Furthermore, storage duration of the salads at refrigeration temperatures affected the microbiome, by decreasing the bacterial richness and promoting the dominance of psychrotropic bacteria. Finally, both salads were found to be a reservoir of opportunistic human pathogens, while washing methods usually applied at home proved to be inefficient in their removal.
机译：即食（RTE）绿叶沙拉蔬菜被认为是可以在销售点立即食用而无需进一步处理的食品。这项研究的目的是调查食用RTE色拉的细菌群落组成以及与不同家庭洗涤处理有关的细菌多样性和组成的变化。通过16S rRNA基因高通量测序检查了火箭和菠菜叶片的细菌微生物组。总体上，在沙拉的叶子中检测到886个操作分类单位（OTU）。变形杆菌是最多样化的高级生物分类群，其次是拟杆菌和硬菌。尽管它们在相同的生产设备上进行处理，但火箭显示出与菠菜色拉不同的细菌群落组成，这主要归因于变形杆菌和拟杆菌对总OTU数量的不同贡献。经测试的家庭去污处理方法无法有效地改变两种RTE色拉中的细菌群落组成。此外，色拉在冷藏温度下的储存时间会通过降低细菌的丰富度并增强精神细菌的优势来影响微生物组。最终，两种色拉都被发现是人为致病菌的机会库，而通常在家中使用的洗涤方法被证明不能有效去除它们。

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