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首页> 外文期刊>Pathogens >In silico Identification of Novel Toxin Homologs and Associated Mobile Genetic Elements in Clostridium perfringens
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In silico Identification of Novel Toxin Homologs and Associated Mobile Genetic Elements in Clostridium perfringens

机译：产气荚膜梭菌中新型毒素同源物和相关移动遗传元件的计算机鉴定

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Clostridium perfringens causes a wide range of diseases in a variety of hosts, due to the production of a diverse set of toxins and extracellular enzymes. The C. perfringens toxins play an important role in pathogenesis, such that the presence and absence of the toxins is used as a typing scheme for the species. In recent years, several new toxins have been discovered that have been shown to be essential or highly correlated to diseases; these include binary enterotoxin (BecAB), NetB and NetF. In the current study, genome sequence analysis of C. perfringens isolates from diverse sources revealed several putative novel toxin homologs, some of which appeared to be associated with potential mobile genetic elements, including transposons and plasmids. Four novel toxin homologs encoding proteins related to the pore-forming Leukocidin/Hemolysin family were found in type A and G isolates. Two novel toxin homologs encoding proteins related to the epsilon aerolysin-like toxin family were identified in Type A and F isolates from humans, contaminated food and turkeys. A novel set of proteins related to clostridial binary toxins was also identified. While phenotypic characterisation is required before any of these homologs can be established as functional toxins, the in silico identification of these novel homologs on mobile genetic elements suggests the potential toxin reservoir of C. perfringens may be much larger than previously thought.
机译：由于产生多种毒素和细胞外酶，产气荚膜梭菌会在多种宿主中引起多种疾病。产气荚膜梭菌毒素在发病机理中起重要作用，因此毒素的存在与否被用作该物种的分型方案。近年来，发现了几种新毒素，这些毒素被证明与疾病至关重要或高度相关。这些包括二元肠毒素（BecAB），NetB和NetF。在当前的研究中，来自多种来源的产气荚膜梭菌分离物的基因组序列分析揭示了几种推定的新型毒素同源物，其中一些似乎与潜在的移动遗传元件有关，包括转座子和质粒。在A型和G型分离物中发现了四个编码与毛孔形成性白细胞素/溶血素家族相关的蛋白质的新型毒素同源物。在来自人类，受污染的食物和火鸡的A型和F型分离物中鉴定出了两种编码与ε-气溶素样毒素家族有关的蛋白质的新型毒素同源物。还鉴定了一组新的与梭菌二元毒素有关的蛋白质。虽然在将任何这些同源物确定为功能毒素之前都需要对表型进行表征，但是在移动遗传元件上对这些新的同源物进行计算机鉴定表明，产气荚膜梭菌的潜在毒素库可能比以前认为的要大得多。

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