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首页> 外文期刊>Pharmacognosy Research >High-performance Thin-layer Chromatography Method Development, Validation, and Simultaneous Quantification of Four Compounds Identified in Standardized Extracts of Orthosiphon stamineus
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High-performance Thin-layer Chromatography Method Development, Validation, and Simultaneous Quantification of Four Compounds Identified in Standardized Extracts of Orthosiphon stamineus

机译：高效薄层色谱方法的开发，验证和同时定量鉴定邻地菜豆的标准化提取物中的四种化合物

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Context: Orthosiphon stamineus is a medicinal herb widely grown in Southeast Asia and tropical countries. It has been used traditionally as a diuretic, abdominal pain, kidney and bladder inflammation, gout, and hypertension. Aims: This study aims to develop and validate the high-performance thin layer chromatography (HPTLC) method for quantification of rosmarinic acid (RA), 3'-hydroxy-5,6,7,4'-tetramethoxyflavone (TMF), sinensitin (SIN) and eupatorin (EUP) found in ethanol, 50% ethanol and water extract of O. stamineus leaves. Materials and Methods: HPTLC method was conducted using an HPTLC system with a developed mobile phase system of toluene: ethyl acetate: formic acid (3:7:0.1) performed on precoated silica gel 60 F254 TLC plates. The method was validated based on linearity, accuracy, precision, limit of detection, limit of quantification (LOQ), and specificity, respectively. The detection of spots was observed at ultraviolet 254 nm and 366 nm. Results: The linearity of RA, TMF, SIN, and EUP were obtained between 10 and 100 ng/spot with high correlation coefficient value (R2) of more than 0.986. The limit of detection was found to be 122.47 ± 3.95 (RA), 43.38 ± 0.79 (SIN), 17.26 ± 1.16 (TMF), and 46.80 ± 1.33 ng/spot (EUP), respectively. Whereas the LOQ was found to be 376.44 ± 6.70 (RA), 131.45 ± 2.39 (SIN), 52.30 ± 2.01 (TMF), and 141.82 ± 1.58 ng/spot (EUP), respectively. Conclusion: The proposed method showed good linearity, precision, accuracy, and high sensitivity. Hence, it may be applied in a routine quantification of RA, SIN, TMF, and EUP found in ethanol, 50% of ethanol and water extract of O. stamineus leaves. SUMMARY HPTLC method provides rapid estimation of the marker compound for routine quality control analysis. The established HPTLC method is rapid for qualitative and quantitative fingerprinting of Orthosiphon stamineus extract used for commercial product. Four identified markers (RA, SIN, EUP and TMF) found in three a different type of O. stamineus extracts specifically ethanol, 50% ethanol and water extract were successfully quantified using HPTLC method. Abbreviations Used : HPTLC: High-performance thin layer chromatography; RA: Rosmarinic acid; TMF: 3’-hydroxy-5,6,7,4’-tetramethoxyflavone; SIN: Sinensitin; EUP: Eupatorin; E: Ethanol; EW: 50% ethanol; W: Water; BK: Batu Kurau; KB: Kepala Batas; S: Sik; CJ: Changkat Jering; SB: Sungai Buloh Key words: Eupatorin, high-performance thin-layer chromatography, Orthosiphon stamineus , rosmarinic acid, sinensitin, validation.
机译：背景：菊科Orthosiphon stamineus是一种在东南亚和热带国家广泛种植的药用草药。传统上，它被用作利尿剂，腹痛，肾脏和膀胱发炎，痛风和高血压。目的：本研究旨在开发和验证用于定量测定迷迭香酸（RA），3'-羟基-5,6,7,4'-四甲氧基黄酮（TMF），sinensitin（RA）的高性能薄层色谱（HPTLC）方法。 SIN）和eupatorin（EUP）存在于乙醇，拟南芥叶片的50％乙醇和水提取物中。材料和方法：HPTLC方法是使用HPTLC系统进行的，该系统具有在预涂硅胶60 F254 TLC板上进行的甲苯，乙酸乙酯：甲酸（3：7：0.1）发达的流动相系统。分别基于线性，准确性，精密度，检测限，定量限（LOQ）和特异性验证了该方法。在紫外254nm和366nm处观察到斑点的检测。结果：RA，TMF，SIN和EUP的线性在10至100 ng / spot之间，相关系数值（R 2 ）大于0.986。发现的检出限分别为122.47±3.95（RA），43.38±0.79（SIN），17.26±1.16（TMF）和46.80±1.33 ng / spot（EUP）。 LOQ分别为376.44±6.70（RA），131.45±2.39（SIN），52.30±2.01（TMF）和141.82±1.58 ng / spot（EUP）。结论：该方法具有良好的线性度，精密度，准确性和高灵敏度。因此，它可用于常规定量分析乙醇，50％乙醇和拟南芥叶片的水提取物中发现的RA，SIN，TMF和EUP。发明内容HPTLC方法为常规质量控制分析提供了标记化合物的快速估计。建立的HPTLC方法可快速用于定性和定量指纹图谱，用于商业产品中的菊苣提取物。使用HPTLC方法成功定量了三种不同类型的稻草提取物中发现的四个已鉴定标记（RA，SIN，EUP和TMF），特别是乙醇，50％乙醇和水提取物。所用缩写：HPTLC：高效薄层色谱法; RA：迷迭香酸; TMF：3′-羟基-5,6,7,4′-四甲氧基黄酮;罪恶：Sinensitin; EUP：Eupatorin; E：乙醇; EW：50％乙醇; W：水; BK：巴图库劳; KB：Kepala Batas; S：ik CJ：Changkat Jering; SB：Sungai Buloh关键词：Eupatorin，高效薄层色谱法，正邻氨基苯甲酸，迷迭香酸，sinensitin，验证。

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