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Comparative proteome analysis of abdominal adipose tissues between fat and lean broilers

机译：脂肪和瘦肉鸡腹部脂肪组织的蛋白质组比较分析

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Background The molecular mechanism underlying broiler fat deposition is still poorly understood. Method Currently, we used two-dimensional gel electrophoresis (2DE) to identify differentially expressed proteins in abdominal adipose tissues of birds at 4?week of age derived from Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). Results Thirteen differentially expressed protein spots were screened out and identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching against the NCBInr database. These identified proteins were apolipoprotein A-I (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein beta-1 (HSPβ1) and two predicted proteins. These differentially expressed proteins are involved mainly in lipid metabolism, amino acid metabolism, signal transduction, energy conversion, antioxidant, and cytoskeleton. Differential expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were further confirmed by Western blot analysis. Quantitative real-time RT-PCR analyses showed that, of these 13 differentially expressed proteins, only PPIase FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level between the two lines. Conclusions Our results have provided further information for understanding the basic genetics control of growth and development of broiler adipose tissue.
机译：背景技术对肉鸡脂肪沉积的分子机制仍知之甚少。方法目前，我们使用二维凝胶电泳（2DE）鉴定从东北农业大学肉鸡品系中选择不同腹部脂肪含量（NEAUHLF）的4周龄禽的腹部脂肪组织中差异表达的蛋白质。结果通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）筛选并鉴定出13个差异表达的蛋白斑点。通过搜索NCBInr数据库，将蛋白质斑点与13种蛋白质匹配。这些鉴定出的蛋白质是载脂蛋白AI（Apo AI），细胞角蛋白otokeratin，ATP合酶亚基α，肽基脯氨酰顺反异构酶FKBP4（PPIase FKBP4），天冬氨酸转氨酶，碳酸酐酶II（CA-II），前列腺素-H2 D-异构酶前体，纤维蛋白原α链，lamin-A（LMNA），超氧化物歧化酶[Mn]（MnSOD），热激蛋白β-1（HSPβ1）和两个预测的蛋白。这些差异表达的蛋白质主要涉及脂质代谢，氨基酸代谢，信号转导，能量转换，抗氧化剂和细胞骨架。通过蛋白质印迹分析进一步证实了Apo A-1，PPIase FKBP4和细胞角蛋白otokeratin蛋白的差异表达。实时定量RT-PCR分析表明，在这13种差异表达的蛋白质中，只有PPIase FKBP4和细胞角蛋白otokeratin在这两株系之间的mRNA水平差异表达。结论我们的结果为了解肉鸡脂肪组织生长发育的基本遗传学控制提供了进一步的信息。

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