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首页> 外文期刊>Psychiatry and Clinical Psychopharmacology >Evaluation of phosphatidylethanol by ELISA for detection of excessive alcohol use compared with traditional biomarkers: a case-control study
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Evaluation of phosphatidylethanol by ELISA for detection of excessive alcohol use compared with traditional biomarkers: a case-control study

机译：与传统生物标志物相比，通过ELISA评价磷脂酰乙醇的过量酒精使用检测：病例对照研究

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Objective: The highly sensitive chromatographic methods for quantifying phosphatidylethanol (PEth) require high levels of expertice and expensive instrumentation. Enzyme-linked immunosorbent assay (ELISA) kits have been developed for research purposes, but the implementation of PEth immunoassays to screen alcohol consumption has not been applied to the analysis of clinical samples. Our aim was to examine the ELISA method for PEth analysis in clinical samples.Methods: We examined the alterations of the PEth serum levels of 22 male inpatients diagnosed with alcohol dependence according to the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, during alcohol withdrawal (at days 1, 7, and 14) compared to 32 healthy controls using ELISA. All patients were admitted for detoxification treatment at the Alcohol and Substance Addiction Treatment and Rehabilitation Center, Trakya University School of Medicine, Edirne, Turkey. Control subjects were assessed with an initial clinical interview and screened with the Alcohol Use Disorder Identification Test (AUDIT), and they included 16 nondrinkers (AUDIT score?=?0) and 16 social drinkers (AUDIT score??8). We examined the diagnostic accuracy of PEth compared to the traditional biomarkers according to the receiver operating characteristic curve analysis.Results: The patients undergoing detoxification had higher baseline PEth levels than the non-drinkers and social drinkers; the difference between groups showed a marginal trend towards significance (p ?=?0.052). PEth was correlated with the self-reported drinking amount in the past month and AUDIT scores, and the correlations showed marginal trends towards significance (r_(s) ?=?0.269, p ?=?0.049; and r_(s) ?=?0.266, p ?=?0.052; respectively). The PEth levels were statistically significantly correlated with gamma-glutamyl transferase (GGT) (r_(s) ?=?0.355, p ?=?0.010), the correlations with alanine aminotransferase (ALT) and aspartate aminotransferase (AST) trended towards statistical significance (r_(s) ?=?0.230, p ?=?0.095; and r_(s) ?=?0.261, p ?=?0.056, respectively), and PEth was not statistically significantly correlated with mean corpuscular volume (MCV) (r_(s) ?=?0.100, p ?=?0.478). PEth levels decreased statistically significantly during alcohol withdrawal (p ?=?0.002). PEth levels of the nondrinkers and social drinkers did not differ statistically significantly (p ?=?1.000). The area under the curve (AUC) for PEth measured by ELISA was statistically signi?cantly higher than 0.5 (AUC?=?0.691, p ?=?0.024), but PEth had poorer diagnostic efficacy than GGT (AUC?=?0.933, p ??0.001), AST (AUC?=?0.931, p ??0.001), MCV (AUC?=?0.803, p ??0.001), and ALT (AUC?=?0.789, p ??0.01).Conclusions: The AUC of 0.69 shows that the diagnostic accuracy of the assay was poor, regardless of a statistical comparison to 0.5. The use of serum might have led to low concentrations that have not differed much between heavy drinkers and social drinkers or abstainers. Whole blood ELISA implementation for the quantification of PEth may increase its diagnostic efficacy.
机译：目的：定量磷脂酰乙醇（PEth）的高灵敏度色谱方法需要高水平的专业知识和昂贵的仪器。已经开发了酶联免疫吸附测定（ELISA）试剂盒用于研究目的，但是用于筛选酒精消耗的PEth免疫测定的实施尚未应用于临床样品的分析。我们的目的是检验临床样本中PEth分析的ELISA方法。方法：我们根据《精神障碍诊断和统计手册》（第四版）在酒精中毒期间检查了22名诊断为酒精依赖的男性住院患者PEth血清水平的变化与使用ELISA的32位健康对照相比，在第1、7和14天停药的情况比较。所有患者均在土耳其埃迪尔内特拉基亚大学医学院酒精与药物成瘾治疗和康复中心接受了排毒治疗。对照受试者经过初步临床访谈评估，并通过酒精使用障碍识别测试（AUDIT）进行筛查，其中包括16名非饮酒者（AUDIT得分？= 0）和16名社交饮酒者（AUDIT得分？<？8）。根据受试者的工作特征曲线分析，我们检验了PEth与传统生物标志物的诊断准确性。结果：接受排毒的患者的基线PEth水平高于非饮酒者和社交饮者。两组之间的差异显示出显着的显着趋势（p = 0.052）。 PEth与过去一个月的自我报告的饮酒量和AUDIT得分相关，并且相关性显示出显着的显着性趋势（（s）== 0.269，> = 0.049;和< i＞r_s≤0.266，p≤0.052。 PEth水平与γ-谷氨酰转移酶（GGT）有统计学显着相关性（r_（s）α=？0.355，> p？=？0.010），与丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（ AST）趋向于统计显着性（ r_（s）≥0.230， p≥0.095;而 r_（s）≥0.261， p≥0.056，分别与PEth和平均红细胞体积（MCV）无显着相关性（r_（s）≥0.100，p≥0.478）。在戒酒期间，PEth水平在统计学上显着下降（p = 0.002）。非饮酒者和社交饮酒者的PEth水平在统计学上没有显着差异（p = 1.000）。 ELISA测定的PEth的曲线下面积（AUC）统计学上显着高于0.5（AUC？=？0.691，p？=？0.024），但PEth的诊断功效比GGT（AUC？= 0.933，p ＜0.001，AST（AUC ＝ 0.931，p ＜0.001），MCV（AUC ＝ 0.803，p ＜0.001，p）。 ALT（AUC≥0.789，p≤0.01）。结论：AUC为0.69表明，该方法的诊断准确性较差，而与统计学上的0.5比较无关。血清的使用可能导致低浓度，而重度饮酒者与社交饮酒者或弃权者之间的差异不大。全血ELISA用于定量PEth可能会提高其诊断功效。

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