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Effect of the precursor addition on the anticancer alkaloid production using experimental design methodology

机译:使用实验设计方法添加前体对抗癌生物碱生产的影响

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The effect of precursors on the anticancer alkaloid production by submerged fermentation using M. anisopliae 3935 was studied, according to complete experimental design 22 with three central points. The results showed that lysine was the most important variable, however, when both lysine and glucose were added to the fermentation medium, the alkaloid production reached, approximately, 17 mg L-1 after 120 hours of fermentation. Then, the scale-up of the process was carried out and these results were confirmed. Finally, 35 mg L-1 of alkaloid at 192 h were attained after increment of added aminoacid lysine.
机译:根据具有三个中心点的完整实验设计22,研究了前体对使用anisopliae 3935进行深层发酵产生的抗癌生物碱的影响。结果表明,赖氨酸是最重要的变量,但是,在发酵培养基中同时添加赖氨酸和葡萄糖时,发酵120小时后生物碱产量达到约17 mg L-1。然后,进行了工艺放大,并证实了这些结果。最后,增加氨基酸赖氨酸后,在192 h达到了35 mg L-1生物碱。

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