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首页> 外文期刊>Phytochemical Analysis >Optimisation of supercritical fluid extraction of indole alkaloids from Catharanthus roseus using experimental design methodology--comparison with other extraction techniques
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Optimisation of supercritical fluid extraction of indole alkaloids from Catharanthus roseus using experimental design methodology--comparison with other extraction techniques

机译：使用实验设计方法优化长春花中吲哚生物碱的超临界流体提取-与其他提取技术相比

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Response surface modelling, using MODDE 6 software for Design of Experiments and Optimisation, was applied to optimise supercritical fluid extraction (SFE) conditions for the extraction of indole alkaloids from the dried leaves of Catharanthus roseus. The effects of pressure (200-400 bar), temperature (40-80pC), modifier concentration (2.2-6.6 vol%) and dynamic extraction time (20-60 min) on the yield of alkaloids were evaluated. The extracts were analysed by high-performance liquid chromatography and the analytes were identified using ion trap-electrospray ionisation-mass spectrometry. The method was linear for alkaloid concentration in the range 0.18-31 og/mL. The limits of detection and quantification for catharanthine, vindoline, vinblastine and vincristine were 0.2, 0.15, 0.1 and 0.08 og/mL and 2.7, 2.0, 1.3 and 1.1 og/g, respectively. The dry weight content of major alkaloids in the plants were compared using different extraction methods, i.e. SFE, Soxhlet extraction, solid-liquid extraction with sonication and hot water extraction at various temperatures. The extraction techniques were also compared in terms of reproducibility, selectivity and analyte recoveries. Relative standard deviations for the major alkaloids varied from 4.1 to 17.5% in different extraction methods. The best recoveries (100%) for catharanthine were obtained by SFE at 250 bar and 80pC using 6.6 vol% methanol as modifier for 40 min, for vindoline by Soxhlet extraction using dichloromethane in a reflux for 16 h, and for 3',4'-anhydrovinblastine by solid-liquid extraction using a solution of 0.5 m sulphuric acid and methanol (3:1 v/v) in an ultrasonic bath for 3 h. Copyright pb 2007 John Wiley & Sons, Ltd.
机译：使用用于实验设计和优化的MODDE 6软件对响应表面进行建模，以优化超临界流体萃取（SFE）条件，以从长春花的干燥叶片中提取吲哚生物碱。评估了压力（200-400 bar），温度（40-80pC），改性剂浓度（2.2-6.6 vol％）和动态提取时间（20-60分钟）对生物碱产量的影响。通过高效液相色谱分析提取物，并使用离子阱-电喷雾电离-质谱法鉴定分析物。该方法对于生物碱浓度在0.18-31 og / mL范围内是线性的。 Catharanthine，vindoline，vinblastine和长春新碱的检测和定量限分别为0.2、0.15、0.1和0.08 og / mL，以及2.7、2.0、1.3和1.1 og / g。使用不同的提取方法（即SFE，索氏提取，超声声波固液提取和不同温度下的热水提取）比较了植物中主要生物碱的干重含量。还比较了提取技术的重现性，选择性和分析物回收率。在不同的提取方法中，主要生物碱的相对标准偏差为4.1％至17.5％。通过在250 bar和80pC下使用6.6体积％甲醇作为改性剂的SFE在250 bar和80pC的条件下获得最佳的回收率（100％），对于使用二氯甲烷在回流下进行索氏提取的Vindoline，回流16 h，以及3'，4'通过在超声波浴中使用0.5 m硫酸和甲醇（3：1 v / v）的溶液进行固液萃取，制得-anhydrovinblastine 3 h。版权所有pb 2007 John Wiley＆Sons，Ltd.

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