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首页> 外文期刊>Ukrainian Biochemical Journal >Proapoptotic properties of total phytohemagglutinine and its individual isolectins in human cell culture 4BL
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Proapoptotic properties of total phytohemagglutinine and its individual isolectins in human cell culture 4BL

机译：总植物血凝素及其单个异凝集素在人细胞培养4BL中的促凋亡特性

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Phytohemagglutinine (PHA) is widely investigated lectin with mitogenic properties. Recently it was shown that PHA is not only cell proliferation inducer, but also has a toxic or cytostatic effect. However concentration dependence and molecular mechanisms of this effect are not enough investigated. To study proapoptotic properties of total phytohemagglutinine and its isolectins in human cell culture of not tumor origin 4BL we observed a change in the frequency of apoptotic cells in the tested cell culture under the influence of the total phytohemagglutinine and erythroagglutinin by the method of specific color luminescent dye. The activation of caspases-3 and -8 and induction of protein Bax expression under the influence of lectins were detected by Western blot analysis. It was revealed that erythroagglutinin induced apoptosis with the highest efficiency compared with leukoagglutinin and total phytohemagglutinine. The induction of apoptosis in human cell culture of not tumor origin 4BL is probably caused by activating caspase-dependent and mitochondrial signalling.
机译：植物血凝素（PHA）是广泛研究的具有促有丝分裂特性的凝集素。最近显示，PHA不仅是细胞增殖的诱导剂，而且具有毒性或细胞抑制作用。然而，浓度依赖性和这种作用的分子机理还没有得到足够的研究。为了研究总植物血凝素及其异凝集素在非肿瘤起源4BL的人细胞培养物中的凋亡特性，我们观察了受试细胞培养物中凋亡细胞频率的变化，这是通过特定颜色发光法在总植物血凝素和赤藓凝集素影响下进行的染料。通过Western印迹分析检测在凝集素影响下caspases-3和-8的活化以及蛋白Bax表达的诱导。结果表明，与白细胞凝集素和总植物血凝素相比，红细胞凝集素诱导凋亡的效率最高。在不是肿瘤起源的4BL的人细胞培养物中诱导凋亡可能是由激活caspase依赖性和线粒体信号传导引起的。

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