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首页> 外文期刊>The Internet Journal of Pharmacology >A study of the anti-ulcer activity of the ethanolic extract of the leaves of Psidium guajava on experimental animal models
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A study of the anti-ulcer activity of the ethanolic extract of the leaves of Psidium guajava on experimental animal models

机译：番石榴叶乙醇提取物对实验动物模型抗溃疡活性的研究

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The aim of the study is to study the anti-ulcer activity of the ethanolic extract of theleaves of Psidium guajava on experimental animal models. Four groups of albino rats weighing 130-180grams were taken for the study (n=6).Group I : control (3%gum acacia 5ml/kg/day orally for 7days).Group II :Experimental control(Aspirin 400mg/kg orally as single dose on 7th day).Group III :Test (Psidium guajava extract 200mg/kg/day orally for 7days and Aspirin 400mg/kg orally on 7th day) and Group IV:Standard(Ranitidine150mg/kg orally for 7days and Aspirin 400mg/kg orally on 7th day).The stomachs of the sacrificed rats were removed and (1)volume of gastric juice (2)ulcer index (3)pepsin activity(4)free acidity(5)total acidity (6)gastric mucus secretion were studied. The ulcer index, pepsin activity, free and total acidity and volume of gastric juice in group III and IV showed significant decrease in comparison to group II whereas there is increase in gastric mucus secretion (p<.01). Sources and support- Animals-Assam Medical College Animal House Drugs and Equipment- Department of Pharmacology,Assam Medical College Introduction Peptic ulcer is a major health problem with multifactorial etiology. The development of gastric ulcer occurs with acid at the breakdown of mucosal defense 1. The prevalence rate of peptic ulcer is approximately 1 in 54 or 1.84% in USA2 .In the last few years,efforts have been taken to identify new antiulcer drugs from natural sources. Plants are the source of certain known anti-ulcer drugs3. Psidium guajava Linn. is a large small evergreen or sub-deciduous tree with smooth pinkish brown bark exfoliating in thin flakes. Leaves are opposite,2-5.6 inch long, oblong or elliptic oblong and is faintly aromatic, lateral nerves prominent, petiole upto 0.3 inch long. Flowers -1.5 inch across white on 1-3 flowered axillary peduncles, calyx urceolate, lower portion adnate to the ovary,upper portion free and irregularly lobed. Flowers and fruits almost throughout the year but flowers mainly hot season and fruits rainy and cold season4. The leaves of Psidium guajava is known to be useful in stomach upsets, gastric disorders,gastroenteritis5 and in gastric ulcer6. Guava is rich in tannins, phenols, triterpenes, flavonoids, essential oils, saponins, carotenoids, lectins, vitamins, fiber and fatty acids. . The leaves of guava are rich in flavonoids, in particular, quercetin. Guava also has antioxidant properties which is attributed to the polyphenols found in the leaves5. Literature reviews indicated that the ulcer protective activity of this plant has not been evaluated so far. Thus, the aim of the study was to evaluate the protective role of P. guajava on ulceration induced by aspirin on albino rats. Materials and methodsCollection,identification and extraction of plant materials-Fresh tender leaves of P. guajava, approximately one kilogram collected in the month of April-May ,2008 were used for the study. The plant was authenticated by Dr.M.Islam, Professor of Life Science, Dibrugarh University , Assam, India. The plant material was air dried at room temperature. The dried leaves were grounded to fine powder and stored in air tight container. Preparation of extract- The powder obtained was soaked in ethanol for 24 hours in percolator. After 24 hours it was allowed to percolate slowly and extract was collected in Petri dishes7. The extract was concentrated in vacuum using rotary flash evaporator.Animals- The experiments were carried out in albino rats of the species Rattus norvegicus of either sex weighing 130-180 gms. The animals were procured from Chakraborty Enterprise,Kolkata. The study was conducted in accordance with CPCSEA(Committee for the Purpose of control and supervision of Experiment on Animals) guidelines and the study was approved by the Institutional Animal Ethical Committee(Registration no.-634/02/a/CPCSEA). The animals were acclimatized for one week under laboratory conditions. They were fed with standard diet and
机译：该研究的目的是研究番石榴叶的乙醇提取物对实验动物模型的抗溃疡活性。取四组体重为130-180克的白化病大鼠（n = 6）。第一组：对照组（3％阿拉伯树胶5ml / kg /天，口服7天）;第二组：实验对照组（阿司匹林400mg / kg口服）第三组：试验（番石榴提取物200mg / kg /天，口服7天，阿司匹林400mg / kg，第七天口服）和第四组：标准组（雷尼替丁150mg / kg，口服7天，阿司匹林400mg /天）在第7天口服），取出被处死的大鼠的胃，（1）胃液量（2）溃疡指数（3）胃蛋白酶活性（4）游离酸度（5）总酸度（6）胃粘液分泌研究。与第二组相比，第三和第四组的溃疡指数，胃蛋白酶活性，游离和总酸度以及胃液的体积均显着降低，而胃粘液的分泌却有所增加（p <0.01）。来源和支持-动物-阿萨姆医学院动物舍药物和设备-阿萨姆医学院药理学系简介消化性溃疡是多因素病因的主要健康问题。胃溃疡的发生是由于酸在粘膜防御系统的破坏下发生的。在美国，消化性溃疡的患病率约为54分之一，即1.84％。近几年来，人们一直在努力从天然药物中鉴定新的抗溃疡药物。资料来源。植物是某些已知的抗溃疡药物的来源3。番石榴番石榴。是一棵大的常绿或小落叶树，树皮光滑，粉红色棕色，剥落成薄片。叶对生，长2-5.6英寸，长圆形或椭圆形长圆形，微弱芳香，侧神经突出，叶柄长0.3英寸。花-1.5英寸，白色，在1-3个花的腋生花序梗上，花萼水杨酸酯，下部贴生于子房，上部游离和不规则裂片。几乎全年都开花结果，但主要开花的季节是炎热的季节，雨季和寒冷的季节则开花4。众所周知，番石榴叶可用于胃部不适，胃部疾病，胃肠炎5和胃溃疡6。番石榴富含单宁，酚，三萜烯，类黄酮，精油，皂苷，类胡萝卜素，凝集素，维生素，纤维和脂肪酸。 。番石榴叶富含类黄酮，尤其是槲皮素。番石榴还具有抗氧化特性，这归因于叶片中发现的多酚5。文献综述表明，迄今为止尚未评估该植物的溃疡保护活性。因此，该研究的目的是评估番石榴假单胞菌对阿司匹林诱导的白化病大鼠溃疡的保护作用。材料与方法植物材料的收集，鉴定和提取-番石榴的新鲜嫩叶，于2008年4月至5月采集约1公斤。该植物已通过印度阿萨姆邦迪布鲁加尔大学生命科学教授M.Islam博士认证。在室温下将植物材料风干。将干燥的叶子磨成细粉，并保存在气密容器中。提取物的制备-将获得的粉末在渗滤器中在乙醇中浸泡24小时。 24小时后，使其缓慢渗滤并将提取物收集在培养皿中7。提取物使用旋转闪蒸器在真空中浓缩。动物-实验是在重约130-180 gms的两性大鼠Rattus norvegicus的白化病大鼠中进行的。这些动物是从加尔各答的Chakraborty Enterprise购买的。该研究是根据CPCSEA（动物实验的控制和监督委员会）指南进行的，并且该研究得到机构动物伦理委员会的批准（注册号-634 / 02 / a / CPCSEA）。在实验室条件下使动物适应一周。他们接受了标准饮食和

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