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首页> 外文期刊>The Korean Journal of Parasitology >Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis
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Genetic Diversity and Phylogenetic Analysis of the Iranian Leishmania Parasites Based on HSP70 Gene PCR-RFLP and Sequence Analysis

机译：基于HSP70基因PCR-RFLP和序列分析的伊朗利什曼原虫的遗传多样性和系统发育分析

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摘要

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.
机译：尽管利什曼病在伊朗人和动物中分布广泛，在全国范围内对致病因素的遗传特征知之甚少。本研究应用HSP70 PCR-RFLP和序列分析，旨在评估利什曼原虫 spp的遗传多样性和系统发育关系。分离自伊朗地方病灶和可用参考菌株。使用PCR-RFLP和序列分析法对全国几乎所有地区的36种利什曼原虫分离株进行了HSP70基因遗传分析。将原始的HSP70基因序列与从NCBI检索到的同源 Leishmania 序列进行比对，并进行系统进化分析。还估计了遗传多样性的基本参数。 HSP70 PCR-RFLP表现出3种不同的电泳模式，没有进一步的种内变异，对应于该国3种利什曼原虫。 tropica，L。major 和 L。婴儿。系统发育分析显示5个主要进化枝，对应5种物种。伊朗血统，包括 L。 major，L. tropica 和 L。婴儿，分布在三个复合物 L中。 major，L. tropica 和 L。多诺瓦尼。但是，在 L之内。主要和 L。在donovani 物种复合物中，HSP70的系统发育不能清楚地区分 L。主要和 L。 turanica 分离物，位于 L之间。婴儿， L。 donovani 和 L。 chagasi 分离株。我们的结果表明，HSP70 PCR-RFLP和序列分析均是医学上可用于鉴定伊朗患者利什曼原虫种类的工具。然而，靶基因遗传多样性的降低使得不可避免的是，其系统进化只解析主要群体，即物种复合体。

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