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首页> 外文期刊>The Korean Journal of Physiology & Pharmacology >Water Extract of Samultang Reduces Apoptotic Cell Death by H2O2-Induced Oxidative Injury in SK-N-MC Cells
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Water Extract of Samultang Reduces Apoptotic Cell Death by H2O2-Induced Oxidative Injury in SK-N-MC Cells

机译：Samultang的水提取物通过H2O2诱导的SK-N-MC细胞氧化损伤减少凋亡细胞死亡

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The purpose of this study was to evaluate the effects of the water extract of Samultang (SMT), a Chinese herb, on apoptotic cell death by H2O2-induced oxidative stress in SK-N-MC cells. A nuclear fragmentation was observed via fluorescence imaging 12 h after exposure to 30 μM H2O2 and DNA laddering was detected via agarose electrophoresis gel. In addition, increases in sub-G1 phase and cleavage of the PARP protein were observed. However, treatment with SMT for 2 h prior to H2O2 exposure significantly reduced apoptotic cell death induced by incubation with 30 μM H2O2 in SK-N-MC cells. Pre-incubation with water extract of SMT for 2 h prevented the H2O2-induced decrease in mitochondrial transmembrane potential. SMT also attenuated the increase in caspase-3 activity and the breakdown of PARP protein caused by H2O2-induced oxidative stress. These results suggest that the water extract of SMT provides inhibition of apoptotic cell death against oxidative injury in SK-N-MC cells.
机译：这项研究的目的是评估中药Samultang（SMT）的水提取物对H 2 O 2 诱导的氧化性凋亡细胞死亡的影响。 SK-N-MC细胞中的应激。暴露于30μMH 2 O 2 后12 h，通过荧光成像观察到核碎裂，并通过琼脂糖电泳凝胶检测到DNA梯状化。另外，观察到亚G1期增加和PARP蛋白的切割。然而，在暴露于H 2 O 2 之前2 h用SMT处理可显着降低30μMH 2 O孵育诱导的凋亡细胞死亡。 SK-N-MC单元中的 2 。用SMT的水提取物预孵育2 h可防止H 2 O 2 诱导的线粒体跨膜电位降低。 SMT还减弱了H 2 O 2 诱导的氧化应激引起的caspase-3活性增加和PARP蛋白的降解。这些结果表明SMT的水提取物提供了针对SK-N-MC细胞中的氧化损伤的凋亡细胞死亡的抑制作用。

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