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首页> 外文期刊>The Open Complementary Medicine Journal >Antimicrobial Activity of Urine after Ingestion of Differing Daily Doses ofCranberry Juice Cocktail in Pregnancy: A Pilot Study
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Antimicrobial Activity of Urine after Ingestion of Differing Daily Doses ofCranberry Juice Cocktail in Pregnancy: A Pilot Study

机译：每天摄入不同剂量的蔓越莓汁鸡尾酒后尿液的抗菌活性：一项初步研究。

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The objective of this study is to evaluate in vitro antimicrobial activity of urine from pregnant subjectsfollowing cranberry juice cocktail (CJC) or placebo ingestion against common pathogens causing asymptomaticbacteriuria. This study was conducted as an adjunct to a larger randomized, controlled trial. 4-hour uninfected urinesamples were collected from 28 pregnant women who were randomized to cranberry or placebo in three groups: A. CJC240 ml two times daily (C, C; n=10), B: CJC in the AM, then placebo in the PM (C, P; n=10), C: placebo two times daily(P, P; n=8). The pH of all specimens was adjusted to 7 and filtered. Aliquots were independently inoculated withovernight culture of 102-3 cell/ml each of single strains of E. coli with both type I and type II fimbriae, K. pneumoniae,and C. albicans, and incubated. CFU/ml of each specimen was enumerated by subculture with quantitative plate counts induplicate. There were no differences between groups for any of the pathogens studies based on treatment allocation. Wedemonstrated no differences in direct antimicrobial activity against E. coli, K. pneumonia or C. albicans in the urine ofpregnant based on differing daily cranberry doses. This may be due to beta-error.
机译：这项研究的目的是评估从蔓越莓汁鸡尾酒（CJC）或安慰剂摄入后怀孕受试者尿液对引起无症状细菌尿的常见病原体的体外抗菌活性。这项研究是对较大的随机对照试验的补充。从28名孕妇中随机抽取酸果蔓或安慰剂，分为三组，收集4小时未感染尿液样本：A. CJC240 ml每天两次（C，C; n = 10），B：CJC在AM中，然后在安慰剂中PM（C，P; n = 10），C：安慰剂每天两次（P，P; n = 8）。将所有样品的pH调节至7并过滤。将等分试样分别单独接种具有I型和II型菌毛，肺炎克雷伯菌和白色念珠菌的大肠杆菌单株102-3细胞/ ml的过夜培养物，并孵育。通过亚培养计数每个样品的CFU / ml，一式两份地定量平板计数。基于治疗分配的任何病原体研究，两组之间没有差异。根据每天的蔓越莓剂量不同，Wed表明孕妇尿液中对大肠杆菌，肺炎克雷伯菌或白色念珠菌的直接抗菌活性没有差异。这可能是由于beta错误。

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