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首页> 外文期刊>The open biotechnology journal >Effects of ECM Proteins and Cationic Polymers on the Adhesion and Proliferation of Rat Islet Cells
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Effects of ECM Proteins and Cationic Polymers on the Adhesion and Proliferation of Rat Islet Cells

机译：ECM蛋白和阳离子聚合物对大鼠胰岛细胞黏附和增殖的影响

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The effects of extracellular matrix (ECM) proteins and cationic polymers on the adhesion and proliferation ofrat islet cells, RIN-5F cells, were investigated. ECM proteins of laminin, fibronectin, vitronectin, type I collagen, type IIcollagen, and type IV collagen, and cationic polyelectrolytes of poly(L-lysine) and poly(allylamine) were coated on thewells of polystyrene cell culture plates. Their effects on the adhesion and proliferation of RIN-5F in serum-free and serummediums were compared. The cell number on the laminin-coated surface was the highest among the coated surfaces.Laminin promoted cell adhesion more strongly than did the other ECM proteins and cationic polyelectrolytes. Vitronectin,type IV collagen, and poly(L-lysine) showed moderate effects, but type I collagen and type II collagen did not have anyeffects on adhesion and proliferation compared with the uncoated polystyrene cell culture plate. Fibronectin promoted celladhesion but not cell proliferation. Cationic poly(allylamine) had an inhibitory effect in serum-free medium and for longtermculture in serum medium. The ECM proteins of laminin, vitronectin, and type IV collagen, and cationic poly(Llysine)will be useful for the surface modification and construction of biomaterials and scaffolds for islet cell culture andtissue engineering.
机译：研究了细胞外基质（ECM）蛋白和阳离子聚合物对大鼠胰岛细胞RIN-5F细胞黏附和增殖的影响。层粘连蛋白，纤连蛋白，玻连蛋白，I型胶原蛋白，II型胶原蛋白和IV型胶原蛋白的ECM蛋白以及聚L-赖氨酸和聚烯丙胺的阳离子聚电解质被包被在聚苯乙烯细胞培养板上。比较了它们对无血清和血清培养基中RIN-5F粘附和增殖的影响。层粘连蛋白包被的表面上的细胞数量最高，与其他ECM蛋白和阳离子聚电解质相比，层粘连蛋白更能促进细胞粘附。玻连蛋白，IV型胶原蛋白和聚L-赖氨酸显示中等程度的作用，但与未涂覆的聚苯乙烯细胞培养板相比，I型胶原蛋白和II型胶原蛋白对粘附和增殖没有任何影响。纤连蛋白促进细胞粘附，但不促进细胞增殖。阳离子聚烯丙胺在无血清培养基中和在血清培养基中的长期培养均具有抑制作用。层粘连蛋白，玻连蛋白和IV型胶原蛋白以及阳离子聚赖氨酸的ECM蛋白将用于胰岛细胞培养和组织工程的生物材料和支架的表面修饰和构建。

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