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首页> 外文期刊>Toxins >A Saccharomyces cerevisiae Wine Strain Inhibits Growth and Decreases Ochratoxin A Biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus
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A Saccharomyces cerevisiae Wine Strain Inhibits Growth and Decreases Ochratoxin A Biosynthesis by Aspergillus carbonarius and Aspergillus ochraceus

机译：酿酒酵母葡萄酒菌株抑制生长并降低O曲霉毒素A.碳曲霉和曲霉的生物合成

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The aim of this study was to select wine yeast strains as biocontrol agents against fungal contaminants responsible for the accumulation of ochratoxin A (OTA) in grape and wine and to dissect the mechanism of OTA detoxification by a Saccharomyces cerevisiae strain (DISAABA1182), which had previously been reported to reduce OTA in a synthetic must. All of the yeast strains tested displayed an ability to inhibit the growth of Aspergillus carbonarius both in vivo and in vitro and addition of culture filtrates from the tested isolates led to complete inhibition of OTA production. S. cerevisiae DISAABA1182 was selected and further tested for its capacity to inhibit OTA production and pks (polyketide synthase) transcription in A. carbonarius and Aspergillus ochraceus in vitro. In order to dissect the mechanism of OTA detoxification, each of these two fungi was co-cultured with living yeast cells exposed to yeast crude or to autoclaved supernatant: S. cerevisiae DISAABA1182 was found to inhibit mycelial growth and OTA production in both Aspergilli when co-cultured in the OTA-inducing YES medium. Moreover, a decrease in pks transcription was observed in the presence of living cells of S. cerevisiae DISAABA1182 or its supernatant, while no effects were observed on transcription of either of the constitutively expressed calmodulin and β-tubulin genes. This suggests that transcriptional regulation of OTA biosynthetic genes takes place during the interaction between DISAABA1182 and OTA-producing Aspergilli.
机译：这项研究的目的是选择酿酒酵母菌株作为抗真菌污染物的生物控制剂，该污染物负责引起葡萄和葡萄酒中曲毒素A（OTA）的积累，并通过酿酒酵母菌株（DISAABA1182）剖析OTA的解毒机理。以前有报道说可以减少合成果汁中的OTA。所有测试的酵母菌株均显示出在体内和体外均抑制碳曲霉生长的能力，从测试分离物中添加培养物滤液可完全抑制OTA产生。选择酿酒酵母DISAABA1182，并进一步测试其在体外抑制碳酸钠曲霉和och曲霉中OTA产生和pks（聚酮化合物合酶）转录的能力。为了剖析OTA解毒的机理，将这两种真菌中的每一种都与暴露于酵母粗品或高压灭菌的上清液中的活酵母细胞共培养：发现酿酒酵母DISAABA1182抑制了曲霉菌的菌丝体生长和OTA生成。 -在诱导OTA的YES培养基中培养。此外，在存在酿酒酵母DISAABA1182或其上清液的活细胞的情况下，观察到pks转录的减少，而对组成性表达的钙调蛋白和β-微管蛋白基因的转录均未观察到影响。这表明，OTA生物合成基因的转录调控发生在DISAABA1182与生产OTA的曲霉菌之间的相互作用期间。

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