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Efficacy of three antifungal chemicals on the growth in vitro of Aspergillus ochraceus and Aspergillus carbonarius strains isolated from Spanish barley

机译:三种抗真菌化学品对西班牙大麦分离的曲霉菌和曲霉菌菌株体外生长的疗效

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Barley, the fourth largest cultivated cereal crop in the world is a nutritious cereal. Some moulds can produce great losses in barley crops and synthesize hazardous mycotoxins. Ochratoxin A is, perhaps, the most concerning mycotoxin at present and can be produced by Aspergillus carbonarius and A. ochraceus. Application of chemical fungicides is the most widely used strategy to control fungal infection in cereal crops. A study on the application of fungicides mancozeb, copper oxychloride and sulphur on the growth of ochratoxigenic strains of A. carbonarius and A. ochraceus was performed. Lag phases and growth rates for two strains of these species in barley-meal extract agar were measured for each fungicide at 15 °C and 25°C. The efficacy order was mancozeb > copper oxychloride > sulphur. Response to each chemical depended on the dose and temperature. Effectiveness was higher at 15°C than at 25°C. Mancozeb inhibited fungal growth at 40 mg/l and provided lag phases > 24 days at lower doses. Copper oxychloride at 0.7 g/l at 15°C produced lag phases > 12 days but at 25°C it did not delay fungal growth. Sulphur provided large lag phases at 3-8 g/l at 15°C while at 25°C growth was unaffected up to 8 g/l.
机译:大麦,世界上第第四大栽培谷物作物是一种营养丰富的谷物。一些模具可以在大麦作物中产生巨大的损失,并合成危险的霉菌毒素。 Ochratoxin A可能是目前最多关于霉菌毒素,可以通过曲霉菌和A. Ochraceus生产。化学杀菌剂的应用是可控制谷类作物中真菌感染的最广泛使用的策略。进行了杀菌剂甘油,氯酰氧化铜和硫对A. Carmachius和A.Ochraceus菌株生长的研究。在15℃和25℃下测量每种杀菌剂在大麦餐萃取物琼脂中进行两种菌株的滞后阶段和生长速率。疗效顺序是Mancoozeb>氯氧化铜>硫。对每种化学品的反应依赖于剂量和温度。在15℃下效果高于25℃。 ManCozeb抑制40 mg / L的真菌生长,并在较低剂量下提供滞后阶段> 24天。氯氧化铜在0.7g / L的15℃下产生滞后阶段> 12天,但在25℃下,它没有延迟真菌生长。硫在15℃下为3-8克/升提供大的滞后相,而在25℃下,生长不受影响至8g / l。

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