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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Survey of Dirofilaria immitis in dogs from Sivas Province in the Central Anatolia Region of Turkey
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Survey of Dirofilaria immitis in dogs from Sivas Province in the Central Anatolia Region of Turkey

机译：土耳其中部安纳托利亚地区锡瓦斯省的狗中的丝虫感染调查

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In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All Knott-positive samples were also found positive by both PCR and ELISA. According to ELISA and PCR results, no statistically significant difference was found between the prevalence in males (4.2%) and females (1.5%); dogs 0.7?2 years old (2.5%), 3-5 years old (2.2%); and more than 6 years old (7.1%); or stray (3.7%) and owned (1.7%) dogs (P > 0.05). One of the PCR products was selected for sequence analyses of the 16S small subunit rRNA gene and the obtained sequence was submitted to GenBank (KJ183078). The 16S rRNA sequence exhibited 100% identity to other D. immitis sequences available in GenBank (FJ799911, FJ799917, FJ799916). This research provides the first data on D. immitis infections in dogs from Sivas Province by using the combined identification techniques.
机译：在这项研究中，我们使用显微镜，血清学和分子方法从土耳其中部安那托利亚地区（Sivas）调查了狗中的Dirofilaria炎性感染的患病率，并揭示了相关的危险因素。从306个7个月以上的狗中获得全血和血清样本。聚合酶链反应（PCR）和改进的Knott方法（Knott）用于检测微丝aria病，酶联免疫吸附测定（ELISA）用于D.炎性乳房炎抗原的存在。通过PCR和ELISA检测到狗中D. immitis的患病率为2.9％（9/306），通过Knott检测为1.3％（4/306）。通过PCR和ELISA也发现所有Knott阳性样品均为阳性。根据ELISA和PCR结果，男性（4.2％）和女性（1.5％）的患病率之间无统计学差异。狗0.7？2岁（2.5％），3-5岁（2.2％）;超过6岁（7.1％）;或流浪（3.7％）并拥有（1.7％）的狗（P> 0.05）。选择一种PCR产物用于16S小亚基rRNA基因的序列分析，并将获得的序列提交给GenBank（KJ183078）。 16S rRNA序列与GenBank（FJ799911，FJ799917，FJ799916）中可获得的其他D.这项研究通过使用组合鉴定技术，提供了有关锡瓦斯省犬中D.炎性炎感染的首个数据。

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