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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >Effects of different extenders and additives on liquid storage of Awassi ram semen
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Effects of different extenders and additives on liquid storage of Awassi ram semen

机译：不同增量剂和添加剂对Awassi ram精液储液的影响

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The aim of this study was to evaluate the effects of different extenders and additives on ram semen during liquid storage and to demonstrate the role of oxidative stress parameters on this process. In the present study, ejaculates taken by artificial vagina twice a week from 4 rams during the breeding season were used. They were mixed and used if motility and viability were above 70% and there was a 95% intact acrosome. The semen specimens were diluted by Tris-citrate-glucose (TRIS), Tris-TES (TEST), HEPES-buffered Tyrode lactate (TL-HEPES), and Dulbecco?s phosphate-buffered saline (PBS) extenders supplemented with different additives [centrifuged egg yolk, Equex-STM, bovine serum albumin (BSA), and ethylenediaminetetraacetic acid (EDTA)]. The specimens were stored at 4 °C for up to 96 h and evaluated for motility, membrane integrity, acrosome integrity, mitochondrial membrane potential (MMP), and oxidative stress parameters. At the end of the 96-h storage, the highest sperm motility was 64.2 ± 3.7% (P 0.05) and significant loss of sperm motility and membrane integrity were not detected in extender TEST-3, but the MMP rate significantly declined. Acrosome integrity was not affected by storage time or extender types. BSA and EDTA decreased lipid peroxidation (LPO) and total oxidant status (TOS), but did not positively affect motility or membrane integrity. As a result, TRIS, TEST, and TL-HEPES-3 were observed to provide better protection for ram semen during liquid storage at 4 °C than other extenders. The role of oxidative stress and MMP are considerable in liquid storage.
机译：这项研究的目的是评估液体存储过程中不同的增量剂和添加剂对公羊精液的影响，并证明氧化应激参数在此过程中的作用。在本研究中，使用了繁殖季节每周两次从4只公羊通过人工阴道采集的射精。如果运动性和生存力高于70％，并且有95％的完整顶体，则将它们混合使用。精液标本用柠檬酸葡萄糖（TRIS），Tris-TES（TEST），HEPES缓冲的酪氨酸乳酸（TL-HEPES）和Dulbeccos磷酸盐缓冲盐水（PBS）补充了不同添加剂的稀释液稀释[离心蛋黄，Equex-STM，牛血清白蛋白（BSA）和乙二胺四乙酸（EDTA）]。将标本在4°C下保存长达96小时，并评估其运动性，膜完整性，顶体完整性，线粒体膜电位（MMP）和氧化应激参数。在96小时储存结束时，最高的精子活力为64.2＆pm;。在增量剂TEST-3中未检测到3.7％（P <0.05）和精子活力和膜完整性的明显损失，但MMP率显着下降。顶体完整性不受保存时间或扩展剂类型的影响。 BSA和EDTA降低了脂质过氧化（LPO）和总氧化剂状态（TOS），但没有积极影响运动性或膜完整性。结果，观察到TRIS，TEST和TL-HEPES-3在4°C的液体存储过程中为公羊精液提供了比其他填充剂更好的保护。氧化应激和MMP在液体储存中的作用相当大。

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