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首页> 外文期刊>Virology Journal >Anti-herpes virus activities of bioactive fraction and isolated pure constituent of Mallotus peltatus: an ethnomedicine from Andaman Islands
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Anti-herpes virus activities of bioactive fraction and isolated pure constituent of Mallotus peltatus: an ethnomedicine from Andaman Islands

机译：羊肚菌生物活性成分和分离的纯成分的抗疱疹病毒活性：安达曼群岛的民族药

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Background Viral infections, particularly the infections caused by herpes simplex virus (HSV), represent one of the most serious public health concerns globally because of their devastating impact. The aim of this study was to evaluate the antiviral potential of methanolic crude extract of an ethnomedicine Mallotus peltatus, its active fraction and pure compound, against HSV-1?F and HSV-2?G. Result The cytotoxicity (CC50, the concentration of 50% cellular toxicity), antiviral effective concentration (EC50, the concentration required to achieve 50% protection against virus-induced cytopathic effect), plaque reduction and the selectivity index (SI, the ratio of CC50 and EC50) was determined. Results showed that the crude methanolic extract of M. peltatus possessed weak anti-HSV activity. In contrast, the active fraction A and isolated ursolic acid from fraction A exhibited potent antiherpesvirus activity against both HSV-1 (EC50?=?7.8 and 5.5?μg/ml; SI?=?22.3 and 20) and HSV-2 (EC50?=?8.2 and 5.8?μg/ml, and SI?=?21.2 and 18.97). The fraction A and isolated ursolic acid (10?μg/ml) inhibited plaque formation of HSV-1 and HSV-2 at more than 80% levels, with a dose dependent antiviral activity, compared to acyclovir. The time response study revealed that the anti-HSV activity of fraction A and isolated ursolic acid is highest at 2–5?h post-infection. Moreover, the time kinetics study by indirect immunofluorescence assay showed a characteristic pattern of small foci of single fluorescent cells in fraction A- treated virus infected cells at 2?h and 4?h post-infection, suggesting drug inhibited viral dissemination. Further, the PCR study with infected cell cultures treated with fraction A and isolated ursolic acid at various time intervals, failed to show amplification at 48–72?h, like acyclovir treated HSV-infected cells. Moreover, fraction A or isolated ursolic acid showed no interaction in combination with acyclovir. Conclusion This study revealed that bioactive fraction A and isolated ursolic acid of M. peltatus has good anti-HSV activity, probably by inhibiting the early stage of multiplication (post-infection of 0–5?h), with SI value of 20, suggesting its potential use as anti-HSV agents.
机译：背景技术病毒感染，尤其是单纯疱疹病毒（HSV）引起的感染，由于其破坏性影响，代表了全球最严重的公共卫生问题之一。这项研究的目的是评估一种民族药麦草的甲醇粗提物，其活性级分和纯化合物对HSV-1？F和HSV-2？G的抗病毒潜力。结果细胞毒性（CC 50 ，50％细胞毒性的浓度），抗病毒有效浓度（EC 50 ，达到50％的病毒诱导的细胞病保护作用所需的浓度）效果），噬菌斑减少和选择性指数（SI，CC 50 和EC 50 的比率）被确定。结果表明，粗制的M. peltatus甲醇提取物具有较弱的抗HSV活性。相反，活性级分A和从级分A中分离出的熊果酸对HSV-1（EC 50 α=？7.8和5.5？μg/ ml; SI？=？22.3和20）和HSV-2（EC 50 α=α8.2和5.8μg/ ml，SIα=α21.2和18.97）。与无环鸟苷相比，级分A和分离的熊果酸（10？μg/ ml）以超过80％的水平抑制HSV-1和HSV-2的噬斑形成，并具有剂量依赖性的抗病毒活性。时间响应研究表明，A级组分和分离的熊果酸的抗HSV活性在感染后2–5？h最高。此外，通过间接免疫荧光测定法进行的时间动力学研究表明，在感染后2？h和4？h，用A级病毒处理的病毒感染的细胞中单个荧光细胞的小灶具特征性模式，表明药物抑制了病毒的传播。此外，用A级分处理的感染细胞培养物和分离的熊果酸在不同时间间隔进行的PCR研究未能显示出在48-72？h的扩增，就像用阿昔洛韦处理过的HSV感染的细胞一样。此外，级分A或分离的熊果酸与阿昔洛韦联用均未显示相互作用。结论这项研究表明，象牙分支杆菌的生物活性级分A和分离的熊果酸具有良好的抗HSV活性，可能是通过抑制增殖的早期阶段（感染后0-5h），SI值为20，提示其潜在用途可作为抗HSV药物。

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