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首页> 外文期刊>Zootecnia Tropical >Genetic diversity of Brycon orbignyanus in the semi-natural reproductive system, using the RAPD marker
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Genetic diversity of Brycon orbignyanus in the semi-natural reproductive system, using the RAPD marker

机译：利用RAPD标记在半自然繁殖系统中灰ry的遗传多样性。

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The stock enhancement programs are being used more frequently as methods of fish conservation. However, the genetic monitoring and the reproductive procedures evaluation are necessary to achieve viable and reliable results. The purpose of this study was to analyze the genetic diversity of Brycon orbignyanus used in stock enhancement programs, in the semi-natural reproductive system, with the RAPD marker. Fifteen broodstocks (10 male and 5 female) and 95 larvae of the offspring were analyzed. The semi-natural reproductive system allowed the obtaining of a low mortality of the broodstocks using in the mating and all presented spawing/spermiation. The nine primers used yielded 90 fragments, of which 73,33% were polymorphic. Differences (P <0,05) in the frequency of 17 fragments were observed, without the presence of exclusive fragments. The Shannon genetic diversity index and percentage of polymorphic fragments were lower in the offspring individuals. Genetic similarity was higher in the offspring. The analysis of molecular variance results showed that the major part of the genetic variation is within the groups (81,49%) and not between them (18,51%). The identity and genetic distance among the groups were 0,953 and 0,049; respectively. In spite of the semi-natural reproductive system to allow the decrease of mortality and an appropriate reproductive broodstocks behavior, was decrease of the genetic variability in the offspring for the effect of the small number of broodstocks used during the reproduction and for the low existent genetic variability among them.
机译：种群增加计划被更频繁地用作鱼类养护方法。但是，遗传监测和生殖程序评估对于获得可行和可靠的结果是必要的。这项研究的目的是使用RAPD标记分析在半自然繁殖系统中用于种群增强计划的Brycon orbignyanus的遗传多样性。分析了15只亲鱼（10只雄性和5只雌性）和95只后代幼体。半自然生殖系统使交配和所有出现的繁殖/生精过程中亲虾的死亡率降低。使用的9个引物产生90个片段，其中73.33％是多态性的。观察到17个片段的频率存在差异（P <0.05），没有排他性片段。后代个体的香农遗传多样性指数和多态性片段的百分比较低。后代的遗传相似性更高。分子变异结果的分析表明，遗传变异的主要部分在组内（81.49％），而不是在组间（18.51％）。各组之间的同一性和遗传距离为0953和0049。分别。尽管采用半自然生殖系统可以降低死亡率和适当的繁殖亲体行为，但由于繁殖过程中使用的亲虾数量少和遗传数量低，后代的遗传变异性降低了其中的可变性。

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