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首页> 外文期刊>Danishgah-i Ulum-i Pizishki va Khadamat-i Bihdashti-Darmani Shahid Sadugi Yazd. Majallah >In Vitro and in Silico Evaluation of Antibacterial Effect of Methanolic Extracts of Prangos Ferulacea on Single and Biofilm Structure of Streptococcus Mutans
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In Vitro and in Silico Evaluation of Antibacterial Effect of Methanolic Extracts of Prangos Ferulacea on Single and Biofilm Structure of Streptococcus Mutans

机译：桔梗甲醇提取物对变形链球菌单层和生物膜结构的体外和计算机评价

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Introduction: Investigating efficacy of natural products, especially plant materials in dental caries is of great significance. The present study aimed to evaluate in vitro and in silico anti bacterial effects of methanolic extracts of Prangos ferulacea on single and biofilm structure of streptococcus mutans.Methods: In this experimental study, after collecting the plants and determining their variety, they were air- dried and powdered. Then, the plants were extracted with methanol by the maceration method. In order to determine antibacterial activity as well as antibiofilm activity of the extracts, disk diffusion and micro titer assay were applied respectively. Regarding in silco analysis, the molecular docking was also preformed by iGemdock 2.1 software between Glucosyltransferases and 20 phytochemicals of the mentioned plants. SPSS software was utilized to statistically analyze the study data applying variance and Duncan’s multiple range test.Results: The results of the present study showed that studied extracts specially their roots extract up to250-3000 μg/ml have significant antibacterial effects against the single form of becteria, which lead to inhibition of biofilm structures formation of the tested strain, though it can not damage biofilm structures of the bacteria. In addition, MIC and MBC minimum values were determined in 250-1000 μg/ml and 500-3000 μg/ml spectera respectively. Then, silico analysis confirmed that studied phytochemical compounds, especially α-pinen, Pesoralen and Limonene revealed an appropriate interaction to Glucosyltransferases,  which could be regarded as an inhibitor for this enzyme.Conclusion: According to the results of the current study, P.ferulacea has significant antibacterial effects against single structure of S.mutans. However, it does not produce any significant effects on the biofilms structured from this bacteria.
机译：简介：研究天然产物，尤其是植物材料在龋齿中的功效具有重要意义。本研究旨在评估阿魏酸甲醇提取物对变形链球菌单层和生物膜结构的体外和计算机模拟抗菌作用。方法：在本实验研究中，在收集植物并确定其品种后，将其风干。和粉。然后，通过浸渍法用甲醇提取植物。为了确定提取物的抗菌活性和抗生物膜活性，分别应用了圆盘扩散法和微量滴定法。关于硅胶分析，还通过iGemdock 2.1软件在糖基转移酶和上述植物的20种植物化学物质之间进行了分子对接。结果：本研究结果表明，研究提取物特别是其根提取物高达250-3000μg/ ml时，对单药形式的抗生素具有显着的抗菌作用。细菌，尽管它不能破坏细菌的生物膜结构，但会导致抑制其生物膜结构的形成。另外，MIC和MBC最小值分别在250-1000μg/ ml和500-3000μg/ ml光谱中测定。然后，硅计算机分析证实，所研究的植物化学化合物，特别是α-pine烯，Pesoralen和Limonene揭示了与葡糖基转移酶的适当相互作用，可以将其视为该酶的抑制剂。对变形链球菌的单个结构具有显着的抗菌作用。但是，它不会对由这种细菌构成的生物膜产生任何重大影响。

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