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首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >Identify and sensitivity to antifungal drugs of Candida species causing vaginitis isolated from vulvovaginal infected patients in Sana'a city
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Identify and sensitivity to antifungal drugs of Candida species causing vaginitis isolated from vulvovaginal infected patients in Sana'a city

机译：从萨那市的外阴阴道感染患者中分离出的念珠菌引起阴道炎的抗真菌药的鉴定和敏感性

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Recently, antifungal susceptibility testing has become more important because of the increasing incidence of both fungal infections and antifungal drug resistance. Candida vaginitis is a common fungal infection among adult women and it has been estimated that 75% of all adult women experience at least one period of vulvovaginal candidiasis in their lifetime. Several predisposing factors, such as diabetes mellitus, using contraceptive, pregnancy, and broad-spectrum antibiotics are reported as main risk factors for the infection. While, the main etiologic agent of vulvovaginal candidiasis is Candida albicans, more antifungal resistance has been reported among non-albicans species. The aim of the present study was toidentify Candida species anddeterminesusceptibility patterns of vaginal isolates of Candida to antifungal drugs including, clotrimazole, miconazole, terbinafine,nystatin, itraconazole, fluconazole, ketoconazole, and econazole. In the present study, 150 vulvovaginal isolates of Candida species were investigated for performing identify and susceptibility tests. The isolates were kept in sterile distilled water at 4°C in the medical Microbiology laboratory of Al-thourah hospital. All isolates were re-identified using standard methods, germ tube test, CHROMagar Candida test and microscopic characteristics on corn meal by preparation high media. Identification was based on colonies producing a green coloration on CHROMagar and presence chlamydosporse on corn meal agar test which were presumptively identified as C. albicans. Disk diffusion method was used to evaluate susceptibility patterns. Paper disks containing clotrimazole, miconazole, itraconazole, fluconazole, ketoconazole, econazole, nystatin and terbinafine were applied for susceptibility tests. A total of 141 women were included in the study and yeasts were isolated in 93 (65.95%), of them C. albicans was the most common species among the isolates followed by C. glabrata 18 (12.76%), C. tropicalis 24 (17.02%) and C. krusei 6 (4.25%) that were isolated from vaginal infected patients.In the present study, we founded that 5 isolates of C. krusei were sensitive to ketoconazole, clotrimazole and miconazole. In addition both isolates were resistant to fluconazole, nystatin, econazole and terfinafine. Only 9 isolate of C. tropicalis was sensitive to miconazole and terbinafine and two isolates to clotrimazole. Highest sensitivity of C. albicans to antifungal drugs was seen against miconazole (89 of 93 isolates) followed by, clotrimazole (81), terbinafine (38) and ketoconazole (13) whereas 73 isolates were resistant to fluconazole and econazole antifungals. 17 out of 18 isolates of C. glabrata were resistant to fluconazole, whereas all isolates were sensitive to miconazole. Antifungal sensitivity testing suggests that vaginal isolates of Candida were most sensitive to miconazole, clotrimazole, and terbinafine, and least sensitive to econazole and fluconazole.
机译：近来，由于真菌感染和抗真菌药物耐药性的发病率不断增加，抗真菌药敏试验变得越来越重要。念珠菌性阴道炎是成年女性中常见的真菌感染，据估计，所有成年女性中有75％在其一生中至少经历了一次阴道阴道念珠菌病时期。据报道，使用避孕药具，妊娠和广谱抗生素等几种易患因素是感染的主要危险因素。尽管外阴念珠菌病的主要病因是白色念珠菌，但在非白色念珠菌中也有更多的抗真菌耐药性。本研究的目的是确定念珠菌的种类以及确定念珠菌阴道分离株对抗真菌药（包括克霉唑，咪康唑，特比萘芬，制霉菌素，伊曲康唑，氟康唑，酮康唑和益康唑）的敏感性。在本研究中，对150株念珠菌的外阴阴道分离物进行了鉴定和药敏试验。将分离株在Al-thourah医院的医学微生物实验室中于4°C的无菌蒸馏水中保存。使用标准方法，胚芽管试验，CHROMagar念珠菌试验和玉米粉的微观特征（通过制备高培养基）对所有分离物进行重新鉴定。鉴定的依据是在玉米粉琼脂试验中在CHROMagar上产生绿色且菌落中存在衣原体的菌落，这些菌被假定为白色念珠菌。圆盘扩散法用于评估药敏模式。使用含有克霉唑，咪康唑，伊曲康唑，氟康唑，酮康唑，益康唑，制霉菌素和特比萘芬的纸碟进行药敏试验。该研究共纳入141名妇女，其中分离出93株酵母（占65.95％），其中白色念珠菌是最常见的菌种，其次是光滑念珠菌18个（12.76％），热带念珠菌24个（从阴道感染的患者中分离出17.02％）和克鲁斯6（4.25％）。在本研究中，我们发现克鲁斯克鲁维酵母的5株分离株对酮康唑，克霉唑和咪康唑敏感。另外，两种分离物均对氟康唑，制霉菌素，益康唑和特非那芬有抗药性。仅9种热带念珠菌分离株对咪康唑和特比萘芬敏感，另2种分离株对克霉唑敏感。白色念珠菌对抗真菌药的敏感性最高，对咪康唑（93个分离株），克霉唑（81），特比萘芬（38）和酮康唑（13）具有抵抗力，而73个分离株对氟康唑和益康唑抗真菌药耐药。 18株毛毛梭菌分离株中有17株对氟康唑耐药，而所有分离株均对咪康唑敏感。抗真菌药敏试验表明，念珠菌的阴道分离株对咪康唑，克霉唑和特比萘芬最敏感，对益康唑和氟康唑最不敏感。

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