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首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >Spectrophotometric and chromatographic methods for the simultaneous determination of rutin and ascorbic acid in their pharmaceutical formulation
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Spectrophotometric and chromatographic methods for the simultaneous determination of rutin and ascorbic acid in their pharmaceutical formulation

机译：分光光度法和色谱法同时测定药物制剂中的芦丁和抗坏血酸

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Four different methods namely, first-derivative of ratio spectra, bivariate, thin layer chromatography and high performance liquid chromatography were used to determine rutin and ascorbic acid simultaneously in their pharmaceutical dosage form. The derivative ratio spectra method was based on measuring the peak amplitudes for ascorbic acid at 275 and 286 nm using 20.0μg.ml-1 rutin as a divisor over a concentration range of 1.0 – 10.0 μg.ml- 1 for ascorbic acid, while rutin can be measured directly at 363 nm without interference from ascorbic acid over a concentration range of 4.0-20.0 μg.ml-1 for rutin. Bivariate method is used for simultaneous determination of both drugs over a concentration range of 2.0 – 12.0 and 2.0 – 16.0 μg.ml-1 for ascorbic acid and rutin, respectively. The method was based on measuring the absorbance at the selected wavelengths. A TLC separation with densitometric detection of both drugs was achieved using ethyl acetate: methyl isobutyl ketone: formic acid: glacial acetic acid: water [20:12:2.8:1.2:20, v/v/v/v/v] as developing solvent. The method allowed determination of rutin and ascorbic acid in concentration ranges of 2.0-6.0 and 4.0-8.0 μg.spot-1, respectively. Furthermore, a high performance liquid chromatographic procedure with ultraviolet detection at 270 nm was developed for the separation and determination of the studied drugs using a C8 column over a concentration range of 5.0-40.0 and 0.5 – 10.0 μg.ml-1 for rutin and ascorbic acid, respectively. The mobile phase is composed of ammonium acetate buffer: acetonitrile [80: 20, v/v], pH was adjusted to 6.3 by orthophosphoric acid. The proposed methods were successfully applied for the determination of the studied drugs in their mixtures and in pharmaceutical formulations containing them.
机译：比率谱的一阶导数，双变量，薄层色谱法和高效液相色谱法这四种不同的方法可同时测定其药物剂型中的芦丁和抗坏血酸。导数比光谱法是基于20.0μg.ml-1芦丁作为除数的，在275和286 nm处测量抗坏血酸的峰幅度，抗坏血酸的浓度范围为1.0 – 10.0μg.ml-1，而芦丁芦丁的浓度范围为4.0-20.0μg.ml-1，可直接在363 nm处测量，而不受抗坏血酸的干扰。双变量法可同时测定抗坏血酸和芦丁的浓度范围分别为2.0 – 12.0和2.0 – 16.0μg.ml-1的两种药物。该方法基于在选定波长下测量吸光度。使用乙酸乙酯：甲基异丁基酮：甲酸：冰醋酸：水[20：12：2.8：1.2：20，v / v / v / v / v]进行显影，同时进行了两种药物的光密度检测的薄层色谱分离溶剂。该方法可以测定芦丁和抗坏血酸的浓度范围分别为2.0-6.0和4.0-8.0μg.spot-1。此外，开发了一种在270 nm处具有紫外线检测功能的高效液相色谱程序，用于分离和测定所研究药物，方法是使用C8色谱柱，对芦丁和抗坏血酸的浓度范围为5.0-40.0和0.5 – 10.0μg.ml-1。酸。流动相由乙酸铵缓冲液：乙腈[80:20，v / v]组成，用正磷酸将pH调节至6.3。所提出的方法已成功地用于确定所研究药物的混合物和含有它们的药物制剂。

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