Streszczenie Wprowadzenie . Drobnoustroje mikroaerofilne, które rosn? w warunkach zmniejszonej zawarto?ci tlenu s? obecne w jamie ustnej. W?ród nich s? bakterie z rodzaju: Aggregatibacter, Campylobacter, Capnocytophaga, Eikenella, Rothia, Wolinella i Corynebacterium (niektóre gatunki). Te drobnoustroje s? zwi?zane z chorobami przyz?bia i zapaleniem dzi?se?. Wytwarzaj? wiele enzymów, cytotoksyn i czynników wirulencji, które mog? odgrywa? g?ówn? rol? w uszkodzeniu tkanek gospodarza. Wiele bakterii, patogennych dla tkanek przyz?bia, wytwarza ró?ne czynniki, które mog? hamowa? chemotaksj? wieloj?drzastych leukocytów. Leki zio?owe s? cz?sto stosowane profilaktycznie i w leczeniu zaka?eń w obr?bie jamy ustnej. W?ród preparatów jest Salviasept?, który dzia?a przeciwdrobnoustrojowo i przeciwzapalnie. Cel pracy . Oznaczenie aktywno?ci Salviaseptu wobec mikroaerofilnych bakterii. Materia? i metody . Bakterie mikroaerofilne zosta?y wyizolowane z patologicznych kieszonek (19 materia?ów), kana?ów korzeniowych (9) i owrzodzeń jamy ustnej (4). Wra?liwo?? bakterii oznaczono metod? seryjnych rozcieńczeń w agarze Brucella z dodatkiem 5% krwi baraniej. Inoculum zawieraj?ce 105 CFU/kropl? nanoszono na powierzchni? agaru aparatem Steersa. Inkubacj? posiewów prowadzono w anaerostatach (warunki mikroaerofilne) w 37°C przez 48 godz. Za MIC uznano takie najmniejsze st??enie Salviaseptu, które ca?kowicie hamowa?o wzrost bakterii. Wyniki . Z badań wynika, ?e najbardziej wra?liwe na Salviasept by?y szczepy Aggregatibacter (MIC ≤ 0,15–0,62 mg/ml, dla 60% szczepów). Pa?eczki Eikenella i Campylobacter okaza?y si? mniej wra?liwe. Wzrost 40 i 22% szczepów by? hamowany przez st??enia w zakresie ≤ 0,15–0,62 mg/ml. Szczepy nale??ce do rodzaju Capnocytophaga by?y najmniej wra?liwe na Salviasept. Wzrost tych szczepów by? hamowany w st??eniach od 2,5 do > 5,0 mg/ml. Wnioski . Najbardziej wra?liwe na Salviasept by?y szczepy Aggregatibacter, a najmniej wra?liwe szczepy Capnocytophaga. Abstract Background . Microaerophilic bacteria which require decreased oxygen value are present in oral cavity. Among the genus there are Aggregatibacter, Campylobacter, Capnocytophaga, Eikenella, Rothia, Wolinella and Corynebacterium (some of the species). These bacteria are associated with periodontal diseases and gingivitis. They produce enzymes, cytotoxins and virulence factors that play a direct role in the damage of host tissues in the periodontal pockets. Several periodontal pathogens produce illdefined molecules that can inhibit the chemotaxis of polymorphonuclear leucocytes. The herbal drugs are frequently administered prophylactically and for treatment of oral cavity infections. Among the drugs there is Salviasept?, which exhibits, antimicrobial and antiinflammatory properties. Objectives . The aim of the study was to determine the activity of Salviasept against microaerophilic bacteria. Material and Methods . The microaerophilic bacteria were isolated from pathological pockets (19 samples), root canal (9) and oral ulcers (4). The susceptibility of bacteria was determined by means of the agar dilution technique in Brucella agar supplemented with 5% sheep blood. The inoculum of 105 CFU/spot was applied to agar plates with Steers replicator. Incubation was performed in anaerobic jars (microaerophilic conditions) at 37°C for 48 hrs each. The MIC was defined as the lowest concentration that inhibited growth of bacteria. Results . The obtained data showed that the most susceptible to Salviasept were the strains of Aggregatibacter (MIC ≤ 5–0.62 mg/ml for 60% strains). The strains of Eikenella and Campylobacter were less sensitive to the specimen. The growth of 40 and 22% of strains were inhibited by concentrations ≤ 0.15–0.62 mg/ml. The strains of rods belonging to genera Capnocytophaga were the least sensitive to Salviasept. The growth of these strains were inhibited in concentrations 2.5 to > 5.0 mg/ml. Conclusion . The most susceptible to Salviasept were the strains of Aggregatibacter and the least sensitive strains of Capnocytophaga.
展开▼
