首页> 外文期刊>Journal of soils & sediments >Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface layer of flooded paddy field soil
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Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface layer of flooded paddy field soil

机译:微苯胺氧化铁氧化细菌在淹水稻田土壤表面层的铁氧化过程中

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Purpose To reveal whether microaerophilic Fe(II)-oxidizing bacteria (FeOB) participate in the Fe(II) oxidation at the oxic-anoxic interface in flooded paddy field soil, distribution of microaerophilic FeOB belonging toGallionellaceae(Gallionella-related FeOB) in the surface layer of a flooded paddy soil microcosm and O(2)conditions for the Fe(II) oxidation by a microaerophilic Fe(II) oxidizer,Ferrigenium kumadaiAn22, were investigated. Materials and methods Flooded paddy soil microcosms were incubated for 30 days. Five soil layers were sampled at 2-mm intervals from the soil surface after the incubation. The community structure ofGallionella-related FeOB was analyzed with qPCR and DGGE methods. In the culture experiment, O(2)and Fe(II) profiles inF. kumadaiAn22-inoculated and non-inoculated gel-stabilized gradient tubes were analyzed, in which an opposing gradient of O(2)and Fe(II) was formed. Results A thin oxic layer was formed at the soil surface after the incubation. The copy number of 16S rRNA genes ofGallionella-related FeOB was highest at the top 0-2 mm layer of the soil. DGGE analysis showed that several bands derived fromGallionella-related FeOB newly appeared at the top soil layer with high intensity. In the culture experiment,F. kumadaiA22 grew at the oxic side of the oxic-anoxic interface with the formation of Fe oxides. Conclusion The present study indicated thatGallionella-related microaerophilic FeOB proliferated in the surface layer of flooded paddy field soil, presumably by oxidizing Fe(II) in the oxic-anoxic interface.
机译:目的揭示微苯胺Fe(ii) - 氧化细菌(Feob)是否参与在洪水稻田土壤中的氧 - 缺氧界面的Fe(II)氧化,在表面上分布归属于Galionellaceae(Gallionella相关的FeoB)研究了微碳酸Fe(II)氧化剂,铁庚尼kumaadaian22的Fe(II)氧化的泛洪水水稻微观微观微观微观微观和O(2)条件。材料和方法淹没了水稻土壤微镜具30天。在孵育后,以2mm间隔以2mm的间隔采样五个土壤层。用QPCR和DGGE方法分析了与Gallionella相关的FeoB的社区结构。在培养实验中,O(2)和Fe(II)型缺口。分析了Kumadaian22接种和非接种的凝胶稳定梯形管,其中形成O(2)和Fe(II)的相对梯度。结果在孵育后在土壤表面形成薄的氧层。在土壤的顶部0-2 mm层中,与Ggallionella相关的FeoB的16S rRNA基因的拷贝数最高。 DGGE分析表明,几个带源自Ggallionella相关的FoOB的条带新出现在高强度的顶部土层。在培养实验中,f。 KumadaIa22在氧缺氧界面的氧侧增长,形成Fe氧化物。结论本研究表明,在淹水稻田土的表面层中增殖的与氧化缺氧界面中的Fe(II)氧化过氧化Fe(II),表明与泛滥稻田土壤的表面层增殖的含有相关的微苯胺Feob。

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