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首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >Cytoprotective Activity of Tribublus terrestris Dry Fruit Extract against Oxidative Stress
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Cytoprotective Activity of Tribublus terrestris Dry Fruit Extract against Oxidative Stress

机译：bu藜干果提取物对氧化应激的细胞保护作用

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Oxidative stress generated in the cells has been reported in a number of diseases such as diabetes, cardiovascular disorders, neurodegenerative diseases M and cancer. Reactive oxygen species interact with almost all the biomolecules ultimately leading to cell death by apoptosis or necrosis. Since polyphenolic compounds such as flavanoids and tannins have radical scavenging activity, they could be effective in alleviating cellular damage caused by oxidative stress. The ethanolic extract of Tribublus terrestris dry fruit contains such polyphenolic compounds. In the present study oxidative stress was induced in neural cell line U-87 MG by exposure to hydrogen peroxide and effect on viability of cells was tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) assay in presence or absence of the extract. Statistical analysis by Analysis of Variance (ANOVA) followed by Tukey-Kramer multiple comparison test (F=82.973, p0.001) showed that the cells cultured with extract had greater viability compared to cells cultured without it in presence of hydrogen peroxide. DNA fragmentation assay was also performed which indicated that the extract could prevent apoptosis, characterized by fragmentation of chromosomal DNA and caused by oxidative stress. Further in-vivo experiments might have to be undertaken to ascertain the potential of the extract as a source of dietary anti-oxidants.
机译：在许多疾病中，例如糖尿病，心血管疾病，神经退行性疾病M和癌症中，已经报道了细胞中产生的氧化应激。活性氧几乎与所有生物分子发生相互作用，最终导致细胞通过凋亡或坏死而死亡。由于多酚类化合物（例如黄酮类化合物和丹宁酸）具有自由基清除活性，因此它们可有效缓解氧化应激引起的细胞损伤。 bu藜干果的乙醇提取物含有这种多酚化合物。在本研究中，通过暴露于过氧化氢在神经细胞系U-87 MG中诱导了氧化应激，并用3-（4,5-Dimethylthiazol-2-yl）-2,5-Diphenyltetrazolium Bromide测试了对细胞活力的影响）（MTT）分析在有无提取物的情况下进行。通过方差分析（ANOVA）然后进行Tukey-Kramer多重比较测试（F = 82.973，p <0.001）进行的统计分析表明，与没有过氧化氢的情况下无提取物培养的细胞相比，提取物培养的细胞具有更高的生存能力。还进行了DNA片段化分析，表明提取物可以防止凋亡，其特征在于染色体DNA片段化和氧化应激引起。可能必须进行进一步的体内实验，以确定提取物作为膳食抗氧化剂来源的潜力。

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