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首页> 外文期刊>The Egyptian Journal of Hospital Medicine >Macrophage Migration Inhibitory Factor in Systemic Lupus Erythematosus and its Expression in Lupus Nephritis
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Macrophage Migration Inhibitory Factor in Systemic Lupus Erythematosus and its Expression in Lupus Nephritis

机译：系统性红斑狼疮巨噬细胞迁移抑制因子及其在狼疮性肾炎中的表达

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Objective: the aim of this study was to access the potential involvement of MIF in SLE, its relationship with corticosteroid dose, also, to measure serum and urinary MIF levels in SLE as well as detecting renal MIF expression in SLE GN. Methods: Serum and urine MIF concentrations were measured by enzyme-linked immunosorbent assay in 20 SLE female patients with lupus nephritis , World Health Organization class II, III, IV, with mean age of 35.1510.42 years and in 10 normal healthy, age matched, female volunteers. All patients were subjected to detailed clinical assessment and laboratory investigations. Serum and urinary MIF concentrations were measured by ELISA technique. Renal MIF expression was assessed by immunostaining of biopsy tissue. Univariate and multivariate regression analysis were used to examine the associations between serum and urine MIF concentrations, renal MIF expression, disease-related indices of SLE and corticosteroid use. Results: A statistically significant 2.98-fold-increase was detected in mean urinary MIF (U MIF) levels in SLE patients compared to controls. While, mean Serum MIF (S MIF) showed no significant difference between cases & control. Both S & U MIF concentrations were positively correlated with SLICC/ACR DI but not with SLEIDAI. Corticosteroid doses showed a highly positive correlation with S MIF, serum creatinine & SLICC/ACR DI. Also a positive correlation was found between the different histopathologic grades of renal affection & the U MIF. Immunohistochemistry staining of all normal kidney specimens showed that MIF is constitutively weakly expressed by some glomerular & parietal epithelial cells & by most tubular epithelial cells. In contrast, there was a significant increase in glomerular & tubular MIF protein staining in SLE nephropathy. This increased MIF expression correlated positively with both S MIF and U MIF, SLICC/ACR DI & the daily steroid dose Conclusion: This study shows that serum MIF is over-expressed in SLE patients and that the urine MIF concentration is significantly increased in SLE World Health Organization class IV patients and correlates with the degree of renal injury. Thus, urine MIF levels reflect MIF expression within the kidney
机译：目的：本研究的目的是了解SLE中MIF的潜在参与及其与皮质类固醇剂量的关系，还可以测量SLE中血清和尿中MIF的水平以及检测SLE GN中肾脏MIF的表达。方法：采用酶联免疫吸附法测定世界卫生组织Ⅱ，Ⅲ，Ⅳ类20例SLE女性狼疮肾病患者的血清和尿中MIF浓度，其平均年龄为35.1510.42岁，年龄为10岁的正常健康者，女性志愿者。所有患者均接受了详细的临床评估和实验室检查。血清和尿中MIF的浓度通过ELISA技术测量。肾MIF表达通过活检组织的免疫染色进行评估。使用单因素和多元回归分析来检查血清和尿液MIF浓度，肾脏MIF表达，疾病相关SLE指数和皮质类固醇使用之间的关联。结果：与对照组相比，SLE患者的平均尿MIF（U MIF）水平增加了2.98倍，具有统计学意义。同时，平均血清MIF（S MIF）在病例与对照组之间没有显着差异。 S和U MIF浓度均与SLICC / ACR DI呈正相关，而与SLEIDAI无正相关。皮质类固醇剂量与S MIF，血清肌酐和SLICC / ACR DI呈高度正相关。在不同的病理病理学分级的肾脏病变和U MIF之间也发现正相关。所有正常肾脏标本的免疫组织化学染色显示，MIF在某些肾小球和顶叶上皮细胞以及大多数肾小管上皮细胞中组成性表达较弱。相反，SLE肾病的肾小球和肾小管MIF蛋白染色显着增加。 MIF表达增加与S MIF和U MIF，SLICC / ACR DI和每日类固醇剂量呈正相关结论：这项研究表明，SLE患者血清MIF过表达，SLE世界中尿MIF浓度显着增加卫生组织IV级患者，并且与肾损伤程度相关。因此，尿中MIF水平反映了肾脏中MIF的表达

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