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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Partial Purification of Hen Egg White Lysozyme by Ethanol Precipitation Method and Determination of the Thermal Stability of Its Lyophilized Form
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Partial Purification of Hen Egg White Lysozyme by Ethanol Precipitation Method and Determination of the Thermal Stability of Its Lyophilized Form

机译：乙醇沉淀法部分纯化鸡蛋清溶菌酶及其冻干形式的热稳定性

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Lysozyme was partially purified from hen egg white by precipitation of non-lysozyme protein impurities during incubation in the presence of ethanol. The thermal stability of the obtained partially purified enzyme was also characterized. The incubation of diluted egg white for 2-8 h in the presence of 20% ethanol was not very effective for the partial purification of lysozyme by precipitation of major egg white proteins; however, 4- to 6-h or 6- to 8-h incubation of diluted egg white in the presence of 30% and 40% ethanol could be employed more effectively for partial purification of lysozyme. Without applying the incubation period, the highest specific activity was obtained by the treatment of egg white with 40% ethanol. Thus, ethanol at this concentration could be used for a continuous process of partial purification. For batch lysozyme purification, on the other hand, incubation in the presence of 30% ethanol was more appropriate. The activities and protein contents of dialyzed and lyophilized enzymes obtained by 6 h-incubation in the presence of 20%, 30%, and 40% ethanol precipitations were 1878, 6669, and 6115 U/mg powder, and 0.98, 0.90, and 0.93 mg protein per mg powder, respectively. The ranges of thermal inactivation parameters, such as D (D80 °C = 29.2-59 min, D90 °C = 8.8-21 min) and z (z80-90 °C = 17.4-22.3 °C) values of the enzyme, clearly indicated the moderate and variable heat stability of lyophilized lysozymes obtained from different batches of egg white.
机译：在乙醇存在下温育期间，通过沉淀非溶菌酶蛋白质杂质，从蛋清中部分纯化了溶菌酶。还对获得的部分纯化的酶的热稳定性进行了表征。在20％乙醇存在下，将稀释的蛋清孵育2-8小时对于通过沉淀主要蛋清蛋白部分纯化溶菌酶不是很有效。然而，在30％和40％乙醇存在下，将稀释蛋清进行4至6小时或6至8小时的孵育可更有效地用于部分纯化溶菌酶。在不应用潜伏期的情况下，通过用40％乙醇处理蛋清可获得最高的比活。因此，该浓度的乙醇可用于部分纯化的连续过程。另一方面，对于分批溶菌酶纯化，在30％乙醇存在下孵育更合适。在20％，30％和40％乙醇沉淀的条件下进行6小时孵育获得的透析和冻干酶的活性和蛋白质含量分别为1878、6669和6115 U / mg粉末，0.98、0.90和0.93毫克蛋白/毫克粉末。酶的热失活参数范围很明显，例如D（D80°C = 29.2-59分钟，D90°C = 8.8-21分钟）和z（z80-90°C = 17.4-22.3°C）值表示从不同批次的蛋白中获得的冻干溶菌酶的中等和可变的热稳定性。

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